A surface-enhanced Raman scattering aptasensor for Escherichia coli detection based on high-performance 3D substrate and hot spot effect.
Anal Chim Acta
; 1221: 340141, 2022 Aug 15.
Article
en En
| MEDLINE
| ID: mdl-35934373
ABSTRACT
A surface-enhanced Raman scattering (SERS) aptasensor was established for highly sensitive and selective detection of Escherichia coli (E. coli). Chitosan hydrogel modified with E. coli aptamer (Apt) functionalized silver nanoparticles was constructed as a SERS 3D substrate for specific bacteria enrichment, while the Raman signaling molecule 4-mercaptobenzoic acid and E. coli Apt modified gold nanostars were prepared for the sensitive quantification of E. coli. The aptasensor exhibits intense electromagnetic field enhancement in multiple hot spot regions, including the spikes and the gap between adjacent nanostars and that between gold nanostars and silver nanoparticles. Due to the hot spot effect coupled with the selective recognition ability, a detection limit of 3.46 CFU/mL with a wide dynamic linearized range from 3.2 × 101 to 3.2 × 107 CFU/mL could be achieved without other non-target bacteria interference. Moreover, this SERS aptasensor was applied to detect E. coli in actual samples with a good recovery rate (>90%). Therefore, the developed SERS aptasensor paves a new avenue for the detection in the field of food safety and environmental pollution by replacing the corresponding aptamers.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Contexto en salud:
3_ND
Problema de salud:
3_neglected_diseases
/
3_zoonosis
Asunto principal:
Técnicas Biosensibles
/
Aptámeros de Nucleótidos
/
Nanopartículas del Metal
Tipo de estudio:
Diagnostic_studies
Idioma:
En
Revista:
Anal Chim Acta
Año:
2022
Tipo del documento:
Article
País de afiliación:
China