Nanofluorescence Probe in Detection of miR-187 and Its Correlation with Oxidative Stress Response in Cataracts.

ABSTRACT

Objective: This study aimed to explore the sensitivity of a nano-fluorescent probe in the detection of miR-187 and the correlation of miR-187 with cataract oxidative stress response. Method: We selected 24 patients with cataracts and 24 healthy people from January 2019 to January 2021 to undergo a nano-fluorescence miR-187 test. We divided cultured human lens epithelial cells (HLECs) into 3 groups control, overexpressed miR-187 and miR-187 silence, in order to investigate the intracellular oxidative stress response, and the activity and apoptosis of HLECs in the state of oxidative stress. Results: The expression of miR-187 increased significantly in patients with cataracts, and the overexpression of miR-187 promoted the oxidative stress response in HLECs. In the oxidative stress environment simulated by hydrogen peroxide, the downregulation of miR-187 can significantly increase HLEC activity and reduce its apoptosis. In order to further study the role of miR-187 in cataract progression, cataract mouse models were injected with miR-187 mimic and inhibitor. The results showed that miR-187-inhibitor can inhibit the progression of cataracts. Conclusion: The expression of miR-187 is significantly related to cataract prognosis, and down regulation of miR-187 expression has significant antioxidation capacity and can reduce HLEC apoptosis.