An ultrasensitive label-free biosensor based on aptamer functionalized two-dimensional photonic crystal for kanamycin detection in milk.
Food Chem
; 402: 134239, 2023 Feb 15.
Article
en En
| MEDLINE
| ID: mdl-36122476
ABSTRACT
A novel photonic crystal aptamer biosensor SiO2-Au-ssDNA two-dimensional photonic crystal (2D PC), allowing label-free and highly sensitive to kanamycin (KANA), is successfully manufactured. This 2D PC biosensor was prepared via a needle tip flow method, using electrostatic adsorption to introduce negatively charged gold nanoparticles (Au NPs) into the 2D PC, combined with sulfhydryl-modified ssDNA for the rapid measurement. Benefiting from the localized surface plasmon resonance effect of Au NPs and optical response capability of PC, the biosensor has an excellent performance on quantitative analysis of KANA ranging from 5 pgâmL-1 to 5 µgâmL-1, with a limit of detection of 1.10 pgâmL-1. The recovery of KANA is between 97 % and 110 % in the milk samples with relative standard deviation less than 4.8 %, which revealing that the 2D PC biosensor has the excellent performance on the KANA detection in complex conditions.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Técnicas Biosensibles
/
Aptámeros de Nucleótidos
/
Nanopartículas del Metal
Límite:
Animals
Idioma:
En
Revista:
Food Chem
Año:
2023
Tipo del documento:
Article