Quantification and characterization of mouse and human tissue-resident microbiota by qPCR and 16S sequencing.
STAR Protoc
; 3(4): 101765, 2022 12 16.
Article
en En
| MEDLINE
| ID: mdl-36209427
ABSTRACT
The tissue-resident microbiota is an integral component of multiple tumor types, but it remains challenging to characterize its abundance and composition due to its low biomass. Here, we describe an optimized protocol for quantification and profiling of tissue-resident microbiota. The major optimized steps include DNA extraction, qPCR, 16S library construction, and bioinformatics analysis. This protocol enables robust and accurate characterization of the dynamics of normal and tumor tissue-resident microbiota at its physiological abundance from both mouse and human origins. For complete details on the use and execution of this protocol, please refer to Fu et al. (2022).
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Microbiota
/
Neoplasias
Límite:
Humans
Idioma:
En
Revista:
STAR Protoc
Año:
2022
Tipo del documento:
Article