A full-length infectious cDNA clone of a dsRNA totivirus-like virus.
Virology
; 576: 127-133, 2022 11.
Article
en En
| MEDLINE
| ID: mdl-36242926
ABSTRACT
Totivirus-like viruses are a group of non-segmented double-stranded (ds)RNA viruses with two open reading frames, which were recently discovered and provisionally assigned to the Totiviridae family. Unlike yeast and protozoan Totiviridae viruses, these totivirus-like viruses infect a diverse spectrum of metazoan hosts and currently have enormous impacts on fisheries and agriculture. We developed the first infectious full-length cDNA clone of a totivirus-like virus, the Omono River virus (OmRV), and produced infectious particles using an RNA-transcript-based method. Compared with the parent wild-type particles from nature, the infectious-cloning OmRV particles have presented strong cytopathic effects, infectivity and similar morphology. Thus far, the established system is one of the few reported systems for generating a non-segmented dsRNA virus cDNA clone.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Totiviridae
/
Totivirus
Límite:
Animals
Idioma:
En
Revista:
Virology
Año:
2022
Tipo del documento:
Article
País de afiliación:
Suecia