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DNA nanostructure-assisted detection of carcinoembryonic antigen with a solid-state nanopore.
Tian, Rong; Weng, Ting; Chen, Shanchuan; Wu, Ji; Yin, Bohua; Ma, Wenhao; Liang, Liyuan; Xie, Wanyi; Wang, Yunjiao; Zeng, Xiaoqing; Yin, Yajie; Wang, Deqiang.
Afiliación
  • Tian R; Chongqing School, University of Chinese Academy of Sciences, Beijing, China; Chongqing Institute of Green and Intelligent Technology, Chinese Academy of Sciences, Beijing, China.
  • Weng T; Chongqing School, University of Chinese Academy of Sciences, Beijing, China; Chongqing Institute of Green and Intelligent Technology, Chinese Academy of Sciences, Beijing, China.
  • Chen S; Chongqing School, University of Chinese Academy of Sciences, Beijing, China; Chongqing Institute of Green and Intelligent Technology, Chinese Academy of Sciences, Beijing, China.
  • Wu J; Chongqing School, University of Chinese Academy of Sciences, Beijing, China; Chongqing Institute of Green and Intelligent Technology, Chinese Academy of Sciences, Beijing, China.
  • Yin B; Chongqing School, University of Chinese Academy of Sciences, Beijing, China; Chongqing Institute of Green and Intelligent Technology, Chinese Academy of Sciences, Beijing, China; Changchun University of Science and Technology, Changchun, China.
  • Ma W; Chongqing School, University of Chinese Academy of Sciences, Beijing, China; Chongqing Institute of Green and Intelligent Technology, Chinese Academy of Sciences, Beijing, China.
  • Liang L; Chongqing School, University of Chinese Academy of Sciences, Beijing, China; Chongqing Institute of Green and Intelligent Technology, Chinese Academy of Sciences, Beijing, China.
  • Xie W; Chongqing School, University of Chinese Academy of Sciences, Beijing, China; Chongqing Institute of Green and Intelligent Technology, Chinese Academy of Sciences, Beijing, China.
  • Wang Y; Chongqing School, University of Chinese Academy of Sciences, Beijing, China; Chongqing Institute of Green and Intelligent Technology, Chinese Academy of Sciences, Beijing, China.
  • Zeng X; Chongqing School, University of Chinese Academy of Sciences, Beijing, China; Chongqing Institute of Green and Intelligent Technology, Chinese Academy of Sciences, Beijing, China; Chongqing University, Chongqing, China.
  • Yin Y; Chongqing School, University of Chinese Academy of Sciences, Beijing, China; Chongqing Institute of Green and Intelligent Technology, Chinese Academy of Sciences, Beijing, China. Electronic address: yinyajie@cigit.ac.cn.
  • Wang D; Chongqing School, University of Chinese Academy of Sciences, Beijing, China; Chongqing Institute of Green and Intelligent Technology, Chinese Academy of Sciences, Beijing, China. Electronic address: dqwang@cigit.ac.cn.
Bioelectrochemistry ; 149: 108284, 2023 Feb.
Article en En | MEDLINE | ID: mdl-36244111
ABSTRACT
In this paper, a novel detection technique for tumor marker carcinoembryonic antigen (CEA) has been developed by using a solid-state nanopore as a tool. The system utilizes the specific affinity between aptamer-modified magnetic Fe3O4 and CEA, rather than directly detecting the translocation of CEA through the nanopore. The aptamer-modified magnetic Fe3O4 was hybridized with tetrahedral DNA nanostructures (TDNs), and TDNs were released after CEA was added. We investigate the translocation behavior of individual TDNs through solid-state nanopores. The frequency of the blockage signals for TDNs is recorded for indirect detection of CEA. We realized the detection of CEA with a concentration as low as 0.1 nM and proved the specificity of the interaction between the aptamer. In addition, our designed nanopore sensing strategy can detect CEA in real samples.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Técnicas Biosensibles / Aptámeros de Nucleótidos / Nanoporos Tipo de estudio: Diagnostic_studies Idioma: En Revista: Bioelectrochemistry Asunto de la revista: BIOQUIMICA Año: 2023 Tipo del documento: Article País de afiliación: China
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Técnicas Biosensibles / Aptámeros de Nucleótidos / Nanoporos Tipo de estudio: Diagnostic_studies Idioma: En Revista: Bioelectrochemistry Asunto de la revista: BIOQUIMICA Año: 2023 Tipo del documento: Article País de afiliación: China