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Effect of Dimethylacetamide Concentration on Motility, Quality, Antioxidant Biomarkers, Anti-Freeze Gene Expression, and Fertilizing Ability of Frozen/Thawed Rooster Sperm.
Mehaisen, Gamal M K; Elomda, Ahmed M; Hamad, Shaimaa K; Ghaly, Mona M; Sun, Yanyan; Li, Yunlei; Zong, Yunhe; Chen, Jilan; Partyka, Agnieszka; Nazmi, Ali; Abbas, Ahmed O; Stino, Farid K R.
Afiliación
  • Mehaisen GMK; Department of Animal Production, Faculty of Agriculture, Cairo University, Giza 12613, Egypt.
  • Elomda AM; Department of Animal Biotechnology, Animal Production Research Institute, Agriculture Research Center, Dokki, Giza 12572, Egypt.
  • Hamad SK; Department of Animal Production, Faculty of Agriculture, Cairo University, Giza 12613, Egypt.
  • Ghaly MM; Department of Animal Sciences, The Ohio State University, Columbus, OH 43013, USA.
  • Sun Y; Department of Animal Production, Faculty of Agriculture, Cairo University, Giza 12613, Egypt.
  • Li Y; Key Laboratory of Animal (Poultry) Genetics Breeding and Reproduction, Ministry of Agriculture and Rural Affairs, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
  • Zong Y; Key Laboratory of Animal (Poultry) Genetics Breeding and Reproduction, Ministry of Agriculture and Rural Affairs, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
  • Chen J; Key Laboratory of Animal (Poultry) Genetics Breeding and Reproduction, Ministry of Agriculture and Rural Affairs, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
  • Partyka A; Key Laboratory of Animal (Poultry) Genetics Breeding and Reproduction, Ministry of Agriculture and Rural Affairs, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
  • Nazmi A; Department of Reproduction and Clinic of Farm Animals, Faculty of Veterinary Medicine, Wroclaw University of Environmental and Life Sciences, 50-375 Wroclaw, Poland.
  • Abbas AO; Department of Animal Sciences, The Ohio State University, Columbus, OH 43013, USA.
  • Stino FKR; Food for Health Discovery Theme, The Ohio State University, Columbus, OH 43013, USA.
Animals (Basel) ; 12(20)2022 Oct 12.
Article en En | MEDLINE | ID: mdl-36290126
ABSTRACT
Sperm cryopreservation is of great importance for the poultry industry but still needs to be optimized. The high susceptibility of poultry sperm to cryodamage leads to low fertility rates after cryopreservation. Therefore, the present study aimed at evaluating the effect of including a cryoprotectant, dimethylacetamide (DMA), in the chicken semen freezing extenders at a final concentration of 3%, 6%, or 9% on the post-thawed sperm motility, quality, antioxidant biomarkers, anti-freeze gene expression, and fertilizing ability. Results showed that the total motile sperm, progressivity, and viability were quadratically increased (p < 0.05) in the 6% DMA group. The antioxidant enzyme activity and lipid peroxidation were negatively (p < 0.05) affected by the increase in DMA concentration. Furthermore, some anti-freeze-associated genes such as heat shock protein 70 (HSP70) and ras homolog family member A (RHOA) were linearly and quadratically down-regulated (p < 0.05) with the high concentration of DMA. Finally, the fertility and hatchability rates did not indicate statistical differences between DMA groups. It can be concluded that using the low concentration of 3−6% DMA in the freezing semen extender is preferable to obtain acceptable results in the post-thawed sperm quality and fertility.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Animals (Basel) Año: 2022 Tipo del documento: Article País de afiliación: Egipto

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Animals (Basel) Año: 2022 Tipo del documento: Article País de afiliación: Egipto
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