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Exosomes from human umbilical cord mesenchymal stem cells protect aortas in Db/db mice characterized by combination of metabolomics and proteomics.
Ling, Ming-Ying; Yang, Xue-Chun; Yu, Na; Song, Yi-Ping; Zheng, Yu-Jing; Tang, Cong-Min; Ding, Wen-Jing; Sun, Yan; Yan, Rong; Wang, Shao-Peng; Li, Xue-Hui; Gao, Hai-Qing; Zhang, Zhen; Xing, Yan-Qiu.
Afiliación
  • Ling MY; Department of Geriatric Medicine, Key Laboratory of Cardiovascular Proteomics of Shandong Province, Qilu Hospital of Shandong University, 250012, Jinan, Shandong, China.
  • Yang XC; Department of Geriatric Medicine, Key Laboratory of Cardiovascular Proteomics of Shandong Province, Qilu Hospital of Shandong University, 250012, Jinan, Shandong, China.
  • Yu N; Shandong Precision Medicine Engineering Laboratory of Bacterial Anti-tumor Drugs, 250101, Jinan, Shandong, China; College of Clinical Medicine, Shandong University, 250012, Jinan, Shandong, China.
  • Song YP; Department of Geriatric Medicine, Key Laboratory of Cardiovascular Proteomics of Shandong Province, Qilu Hospital of Shandong University, 250012, Jinan, Shandong, China.
  • Zheng YJ; Shandong Precision Medicine Engineering Laboratory of Bacterial Anti-tumor Drugs, 250101, Jinan, Shandong, China.
  • Tang CM; Department of Geriatric Medicine, Key Laboratory of Cardiovascular Proteomics of Shandong Province, Qilu Hospital of Shandong University, 250012, Jinan, Shandong, China.
  • Ding WJ; Department of Geriatric Medicine, Key Laboratory of Cardiovascular Proteomics of Shandong Province, Qilu Hospital of Shandong University, 250012, Jinan, Shandong, China.
  • Sun Y; Department of Geriatric Medicine, Key Laboratory of Cardiovascular Proteomics of Shandong Province, Qilu Hospital of Shandong University, 250012, Jinan, Shandong, China.
  • Yan R; Department of Geriatric Medicine, Key Laboratory of Cardiovascular Proteomics of Shandong Province, Qilu Hospital of Shandong University, 250012, Jinan, Shandong, China.
  • Wang SP; Shandong Precision Medicine Engineering Laboratory of Bacterial Anti-tumor Drugs, 250101, Jinan, Shandong, China.
  • Li XH; Department of Geriatric Medicine, Key Laboratory of Cardiovascular Proteomics of Shandong Province, Qilu Hospital of Shandong University, 250012, Jinan, Shandong, China.
  • Gao HQ; Department of Geriatric Medicine, Key Laboratory of Cardiovascular Proteomics of Shandong Province, Qilu Hospital of Shandong University, 250012, Jinan, Shandong, China.
  • Zhang Z; Department of Geriatric Medicine, Key Laboratory of Cardiovascular Proteomics of Shandong Province, Qilu Hospital of Shandong University, 250012, Jinan, Shandong, China. Electronic address: 18560088221@163.com.
  • Xing YQ; Department of Geriatric Medicine, Key Laboratory of Cardiovascular Proteomics of Shandong Province, Qilu Hospital of Shandong University, 250012, Jinan, Shandong, China. Electronic address: xingyanqiu@sina.com.
Arch Biochem Biophys ; 731: 109430, 2022 11 30.
Article en En | MEDLINE | ID: mdl-36326546
Diabetic cardiovascular complication is a common systemic disease with high morbidity and mortality worldwide. We hypothesise that exosomes derived from human umbilical cord mesenchymal stem cells (hUCMSCs-exos) can rescue these disorders and alleviate vascular remodeling in diabetes. Morphological, non-targeted metabolomics and 4D label-free proteomics techniques were used to analyze the aortas of db/m mice as normal control group (NCA), saline treated db/db mice (DMA), and hUCMSCs-exos treated db/db mice (DMTA), and to clarify the molecular mechanism of the protection of hUCMSCs-exos in vascular remodeling from a new point of view. The results showed that 74 metabolites were changed significantly in diabetic aortas, of which 15 were almost restored by hUCMSCs-exos. In proteomics, 30 potential targets such as Stromal cell-derived factor 2-like protein 1, Leukemia inhibitory factor receptor, Peroxisomal membrane protein and E3 ubiquitin-protein ligase MYCBP2 were detected. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway-based analysis showed that Central carbon metabolism in cancer and Galactose metabolism pathway were up-regulated to near normal by hUCMSCs-exos in metabolomics, with janus associated kinase-signal transducer and activator of transcription (JAK-STAT) pathway displayed in proteomics. According to bioinformatics and integrated analysis, these targeted molecules of hUCMSCs-exos to attenuate the vascular remodeling were mainly associated with regulation of energy metabolism, oxidative stress, inflammation, and cellular communications. This study provided a reference for the therapy of diabetes-induced cardiovascular complications.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Exosomas / Células Madre Mesenquimatosas Límite: Animals / Humans Idioma: En Revista: Arch Biochem Biophys Año: 2022 Tipo del documento: Article País de afiliación: China

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Exosomas / Células Madre Mesenquimatosas Límite: Animals / Humans Idioma: En Revista: Arch Biochem Biophys Año: 2022 Tipo del documento: Article País de afiliación: China
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