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Maternal Western-style diet remodels the transcriptional landscape of fetal hematopoietic stem and progenitor cells in rhesus macaques.
Sureshchandra, Suhas; Chan, Chi N; Robino, Jacob J; Parmelee, Lindsay K; Nash, Michael J; Wesolowski, Stephanie R; Pietras, Eric M; Friedman, Jacob E; Takahashi, Diana; Shen, Weining; Jiang, Xiwen; Hennebold, Jon D; Goldman, Devorah; Packwood, William; Lindner, Jonathan R; Roberts, Charles T; Burwitz, Benjamin J; Messaoudi, Ilhem; Varlamov, Oleg.
Afiliación
  • Sureshchandra S; Department of Molecular Biology and Biochemistry, School of Biological Sciences, Institute for Immunology, Center for Virus Research, University of California-Irvine, Irvine, CA 92697, USA.
  • Chan CN; Division of Comparative Medicine, Oregon National Primate Research Center, Beaverton, OR 97006.
  • Robino JJ; Division of Cardiometabolic Health, Oregon National Primate Research Center, Beaverton, OR 97006.
  • Parmelee LK; Division of Comparative Medicine, Oregon National Primate Research Center, Beaverton, OR 97006.
  • Nash MJ; Department of Pediatrics, Section of Neonatology, University of Colorado Anschutz Medical Campus, Aurora, CO 80045, USA.
  • Wesolowski SR; Department of Pediatrics, Section of Neonatology, University of Colorado Anschutz Medical Campus, Aurora, CO 80045, USA.
  • Pietras EM; Department of Immunology and Microbiology, Division of Hematology, University of Colorado Anschutz Medical Campus, Aurora, CO 80045, USA.
  • Friedman JE; Department of Pediatrics, Section of Neonatology, University of Colorado Anschutz Medical Campus, Aurora, CO 80045, USA; Harold Hamm Diabetes Center, University of Oklahoma Health Sciences Center, Oklahoma City, OK 73104, USA.
  • Takahashi D; Division of Comparative Medicine, Oregon National Primate Research Center, Beaverton, OR 97006.
  • Shen W; Department of Statistics, University of California-Irvine, Irvine, CA 92697, USA.
  • Jiang X; Department of Statistics, University of California-Irvine, Irvine, CA 92697, USA.
  • Hennebold JD; Division of Reproductive and Developmental Sciences, Oregon National Primate Research Center, Beaverton, OR 97006; Department of Obstetrics and Gynecology, Oregon Health & Science University, Portland, OR 97239, USA.
  • Goldman D; Stem Cell Center, Oregon Health & Science University, Portland, OR 97239, USA.
  • Packwood W; Knight Cardiovascular Institute, Oregon Health & Science University, Portland, OR 97239, USA.
  • Lindner JR; Division of Cardiometabolic Health, Oregon National Primate Research Center, Beaverton, OR 97006; Knight Cardiovascular Institute, Oregon Health & Science University, Portland, OR 97239, USA.
  • Roberts CT; Division of Cardiometabolic Health, Oregon National Primate Research Center, Beaverton, OR 97006; Department of Pediatrics, Oregon Health & Science University, Portland, OR 97239, USA.
  • Burwitz BJ; Division of Pathobiology and Immunology, Oregon National Primate Research Center, Beaverton, OR 97006; Vaccine & Gene Therapy Institute, Beaverton, OR 97006, USA.
  • Messaoudi I; Department of Molecular Biology and Biochemistry, School of Biological Sciences, Institute for Immunology, Center for Virus Research, University of California-Irvine, Irvine, CA 92697, USA; Department of Immunology, Microbiology and Molecular Genetics, University of Kentucky College of Medicine, Lex
  • Varlamov O; Division of Cardiometabolic Health, Oregon National Primate Research Center, Beaverton, OR 97006. Electronic address: varlamov@ohsu.edu.
Stem Cell Reports ; 17(12): 2595-2609, 2022 12 13.
Article en En | MEDLINE | ID: mdl-36332628
ABSTRACT
Maternal obesity adversely impacts the in utero metabolic environment, but its effect on fetal hematopoiesis remains incompletely understood. During late development, the fetal bone marrow (FBM) becomes the major site where macrophages and B lymphocytes are produced via differentiation of hematopoietic stem and progenitor cells (HSPCs). Here, we analyzed the transcriptional landscape of FBM HSPCs at single-cell resolution in fetal macaques exposed to a maternal high-fat Western-style diet (WSD) or a low-fat control diet. We demonstrate that maternal WSD induces a proinflammatory response in FBM HSPCs and fetal macrophages. In addition, maternal WSD consumption suppresses the expression of B cell development genes and decreases the frequency of FBM B cells. Finally, maternal WSD leads to poor engraftment of fetal HSPCs in nonlethally irradiated immunodeficient NOD/SCID/IL2rγ-/- mice. Collectively, these data demonstrate for the first time that maternal WSD impairs fetal HSPC differentiation and function in a translationally relevant nonhuman primate model.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Células Madre / Dieta Occidental Límite: Animals / Female / Humans / Pregnancy Idioma: En Revista: Stem Cell Reports Año: 2022 Tipo del documento: Article País de afiliación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Células Madre / Dieta Occidental Límite: Animals / Female / Humans / Pregnancy Idioma: En Revista: Stem Cell Reports Año: 2022 Tipo del documento: Article País de afiliación: Estados Unidos