Your browser doesn't support javascript.
loading
Cell mechanical responses to subcellular perturbations generated by ultrasound and targeted microbubbles.
Zhang, Meiru; Zhang, Suyan; Shi, Jianmin; Hu, Yi; Wu, Shuying; Zan, Zhaoguang; Zhao, Pu; Gao, Changkai; Du, Yanyao; Wang, Yulin; Lin, Feng; Fu, Xing; Li, Dachao; Qin, Peng; Fan, Zhenzhen.
Afiliación
  • Zhang M; Department of Biomedical Engineering, Tianjin University, Tianjin 300072, China.
  • Zhang S; Department of Biomedical Engineering, Tianjin University, Tianjin 300072, China.
  • Shi J; School of Sensing Science and Engineering, Shanghai Jiao Tong University, Shanghai 200240, China.
  • Hu Y; Department of Biomedical Engineering, Tianjin University, Tianjin 300072, China.
  • Wu S; Department of Biomedical Engineering, Tianjin University, Tianjin 300072, China.
  • Zan Z; Department of Biomedical Engineering, Tianjin University, Tianjin 300072, China.
  • Zhao P; Department of Biomedical Engineering, Tianjin University, Tianjin 300072, China.
  • Gao C; Department of Biomedical Engineering, Tianjin University, Tianjin 300072, China.
  • Du Y; Department of Biomedical Engineering, Tianjin University, Tianjin 300072, China.
  • Wang Y; Department of Biomedical Engineering, Tianjin University, Tianjin 300072, China.
  • Lin F; Wenzhou Institute, University of Chinese Academy of Sciences, Wenzhou, Zhejiang 325000, China.
  • Fu X; Department of Biomedical Engineering, Tianjin University, Tianjin 300072, China.
  • Li D; Department of Biomedical Engineering, Tianjin University, Tianjin 300072, China.
  • Qin P; School of Sensing Science and Engineering, Shanghai Jiao Tong University, Shanghai 200240, China.
  • Fan Z; Department of Biomedical Engineering, Tianjin University, Tianjin 300072, China; State Key Laboratory of Acoustics, Institute of Acoustics, Chinese Academy of Sciences, Beijing 100190, China. Electronic address: zhenzhen.fan@tju.edu.cn.
Acta Biomater ; 155: 471-481, 2023 01 01.
Article en En | MEDLINE | ID: mdl-36400351
ABSTRACT
The inherently dynamic and anisotropic microenvironment of cells imposes not only global and slow physical stimulations on cells but also acute and local perturbations. However, cell mechanical responses to transient subcellular physical signals remain unclear. In this study, acoustically activated targeted microbubbles were used to exert mechanical perturbations to single cells. The cellular contractile force was sensed by elastic micropillar arrays, while the pillar deformations were imaged using brightfield high-speed video microscopy at a frame rate of 1k frames per second for the first 10s and then confocal fluorescence microscopy. Cell mechanical responses are accompanied by cell membrane integrity changes. Both processes are determined by the perturbation strength generated by microbubble volumetric oscillations. The instantaneous cellular traction force relaxation exhibits two distinct patterns, correlated with two cell fates (survival or permanent damage). The mathematical modeling unveils that force-induced actomyosin disassembly leads to gradual traction force relaxation in the first few seconds. The perturbation may also influence the far end subcellular regions from the microbubbles and may propagate into connected cells with attenuations and delays. This study carefully characterizes the cell mechanical responses to local perturbations induced by ultrasound and microbubbles, advancing our understanding of the fundamentals of cell mechano-sensing, -responsiveness, and -transduction. STATEMENT OF

SIGNIFICANCE:

Subcellular physical perturbations commonly exist but haven't been fully explored yet. The subcellular perturbation generated by ultrasound and targeted microbubbles covers a wide range of strength, from mild, intermediate to intense, providing a broad biomedical relevance. With µm2 spatial sensing ability and up to 1ms temporal resolution, we present spatiotemporal details of the instantaneous cellular contractile force changes followed by attenuated and delayed global responses. The correlation between the cell mechanical responses and cell fates highlights the important role of the instantaneous mechanical responses in the entire cellular reactive processes. Supported by mathematical modeling, our work provides new insights into the dynamics and mechanisms of cell mechanics.
Asunto(s)
Palabras clave

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Microburbujas / Fenómenos Mecánicos Idioma: En Revista: Acta Biomater Año: 2023 Tipo del documento: Article País de afiliación: China

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Microburbujas / Fenómenos Mecánicos Idioma: En Revista: Acta Biomater Año: 2023 Tipo del documento: Article País de afiliación: China
...