Phenotyping polarization dynamics of immune cells using a lipid droplet-cell pairing microfluidic platform.
Cell Rep Methods
; 2(11): 100335, 2022 11 21.
Article
en En
| MEDLINE
| ID: mdl-36452873
ABSTRACT
The immune synapse is the tight contact zone between a lymphocyte and a cell presenting its cognate antigen. This structure serves as a signaling platform and entails a polarization of intracellular components necessary to the immunological function of the cell. While the surface properties of the presenting cell are known to control the formation of the synapse, their impact on polarization has not yet been studied. Using functional lipid droplets as tunable artificial presenting cells combined with a microfluidic pairing device, we simultaneously observe synchronized synapses and dynamically quantify polarization patterns of individual B cells. By assessing how ligand concentration, surface fluidity, and substrate rigidity impact lysosome polarization, we show that its onset and kinetics depend on the local antigen concentration at the synapse and on substrate rigidity. Our experimental system enables a fine phenotyping of monoclonal cell populations based on their synaptic readout.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Microfluídica
/
Gotas Lipídicas
Idioma:
En
Revista:
Cell Rep Methods
Año:
2022
Tipo del documento:
Article
País de afiliación:
Francia