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Flow Characteristics of the Conjugate of Anti-CD3 Monoclonal Antibodies and Magnetic Nanoparticle in PBS and Blood Vessels.
Lee, Sang-Suk; Kim, Eun-Jae; Kang, Mikyung; Kang, Hasung; Choi, Sang-Heon; Jeon, Dain; Choi, Jong-Gu; Choi, Yukyoung; Lee, Hyunsook; Hasan, Mahbub.
Afiliación
  • Lee SS; Department of Oriental Biomedical Engineering, College of Health Sciences, Sangji University, Wonju, Republic of Korea. sslee@sangji.ac.kr.
  • Kim EJ; Department of Medical Laboratory Science, Jeonju Kijeon College, Jeonju, Republic of Korea.
  • Kang M; Interdisciplinary Program for Bioengineering, Seoul National University, Seoul, Republic of Korea.
  • Kang H; Department of Medicine, College of Medicine, Seoul National University, Seoul, Republic of Korea.
  • Choi SH; Department of Oriental Biomedical Engineering, College of Health Sciences, Sangji University, Wonju, Republic of Korea.
  • Jeon D; Department of Oriental Biomedical Engineering, College of Health Sciences, Sangji University, Wonju, Republic of Korea.
  • Choi JG; Department of Oriental Biomedical Engineering, College of Health Sciences, Sangji University, Wonju, Republic of Korea.
  • Choi Y; Department of Oriental Biomedical Engineering, College of Health Sciences, Sangji University, Wonju, Republic of Korea.
  • Lee H; Department of Oriental Biomedical Engineering, College of Health Sciences, Sangji University, Wonju, Republic of Korea.
  • Hasan M; Department of Oriental Biomedical Engineering, College of Health Sciences, Sangji University, Wonju, Republic of Korea.
Adv Exp Med Biol ; 1395: 357-362, 2022.
Article en En | MEDLINE | ID: mdl-36527662
ABSTRACT
Previously, anti-CD3 antibodies delivered intravenously have been known for their negative side effects. The experimental conditions for optimal liquid production are derived from the Fc-directed conjugation of anti-CD3 foralumab antibodies and magnetic nanoparticles (Ab-MNPs). The anti-CD3 antibodies are prepared for conjugation with MNPs using SiteClick antibody labelling kits. The successful conjugation of the Ab-MNPs is confirmed using a transmission electron microscopy (TEM) image and an energy dispersive spectroscopy (EDS) analysis. The average values ​​of the moving speed of MNPs and Ab-MNPs in phosphate buffer saline (PBS) were + 3.16 pix/frame and + 6.70 pix/frame in the x-axis, respectively. This implies that MNPs with CD3 antibodies attached to the surface through biocompatible ligand functional groups has better fluidity in PBS. Afterwards, a non-clinical animal testing for the flow characteristics of Ab-MNPs inside a blood vessel is carried out to observe the effects of Ab-MNP delivery through intravenous injection.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Nanopartículas de Magnetita Límite: Animals Idioma: En Revista: Adv Exp Med Biol Año: 2022 Tipo del documento: Article
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Nanopartículas de Magnetita Límite: Animals Idioma: En Revista: Adv Exp Med Biol Año: 2022 Tipo del documento: Article