Practical approach for optimizing off-axis telecentric digital holographic microscope design.

Digital holographic microscopy (DHM) has become an attractive imaging tool for the analysis of living cells and histological tissues. Telecentric DHM (TDHM) is a configuration of DHM that reduces the computational demands through a priori aberration corrections. However, TDHM requires a well-aligned optical pipeline to optimize its resolution and image quality (IQ), which has traditionally complicated the alignment process. Derived from optical interference functions, we offer here a set of methodologies to simplify TDHM design and alignment by determining the optimal +1-order position, which depends on the object-reference beam angle and the interference plane rotation angle. The methods are then experimentally tested and verified on a TDHM system by imaging living HeLa cells in suspension.