Microfluidic paper-based fluorescence sensor for L-homocysteine using a molecularly imprinted polymer and in situ-formed fluorescent quantum dots.
Talanta
; 255: 124185, 2023 Apr 01.
Article
en En
| MEDLINE
| ID: mdl-36634429
ABSTRACT
Microfluidic paper-based analytical devices modified with molecularly imprinted polymers (µPADs@MIPs) were developed for fluorescent detection of targeted thiols via in situ UV-induced formation of quantum dots (µPADs@MIPs@QDs). The selectivity enhancement by the MIP layer formed on the filter paper surface was demonstrated for the isolation of L-homocysteine from wine. Followed by the addition of metal precursors solution (Zn/Cd/Cu) and UV irradiation, fluorescent quantum dots were formed thus enabling quantitative detection of the thiol (serving as a QD capping agent). The effect of different semiconductors was investigated to achieve a lower band gap and higher fluorescence intensity. Increasing fluorescence intensity in the presence of thiol groups was obtained for the following precursors mixture composition ZnCdCu/S > ZnCd/S > ZnCu/S > ZnS. The proposed method has a good relationship between the fluorescence intensity of ZnCdCu/S QDs and L-homocysteine in a linear range from 0.74 to 7.40 µM with a limit of detection (LOD) and quantification (LOQ) of 0.51 and 1.71 µM respectively. This method was applied for the determination of L-homocysteine in white wine with RSD under 6.37%.
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Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Puntos Cuánticos
/
Impresión Molecular
Idioma:
En
Revista:
Talanta
Año:
2023
Tipo del documento:
Article
País de afiliación:
República Checa