Light-induced fusion of liposomes with release of trapped marker dye is sensitised by photochromic phospholipid.
Biochim Biophys Acta
; 903(3): 504-9, 1987 Oct 16.
Article
en En
| MEDLINE
| ID: mdl-3663656
ABSTRACT
Liposomes have been prepared from dipalmitoylphosphatidylcholine containing small amounts of a synthetic photochromic phospholipid, 'Bis-Azo PC'. In the dark, these are stable at room temperature, and contents do not significantly leak over weeks. Photoisomerisation results in immediate release of trapped marker, and in liposome fusion to form larger structures. Fusion has been detected using a fluorescence polarisation assay, and confirmed by electron microscopy. In mixtures, fusion occurs between 'photochromic' liposomes and those of pure lipid. Bis-Azo PC contains two photochromic acyl chains; analogues bearing a single photochromic chain appear to have little effect on bilayer permeability after isomerisation. Photo-induced leakage and liposome fusion suggest possible applications for localised drug delivery as an adjunct to phototherapy. The ability to non-invasively trigger fusion processes should be useful in fundamental studies of membrane interactions. We believe this to be the first report of photo-induced fusion to date.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Fosfatidilcolinas
/
Luz
/
Liposomas
/
Fusión de Membrana
Idioma:
En
Revista:
Biochim Biophys Acta
Año:
1987
Tipo del documento:
Article
País de afiliación:
Reino Unido