Enzyme-linked immunosorbent assay using thin-layered microfluidics with perfect capture of the target protein.
Anal Methods
; 15(5): 675-684, 2023 02 02.
Article
en En
| MEDLINE
| ID: mdl-36655604
ABSTRACT
We developed a process for enzyme-linked immunosorbent assay on a glass microchip via the use of a thin-layered microfluidic channel. This channel possesses a high aspect ratio (width/depth â¼200) and has an antibody layer immobilized directly on the channel surface. A depth of several microns and an excessive width and length (mm scale) of the channel provide a large-volume capacity (102 nL) and maximum capture efficiency of the analyte for a high level of detection sensitivity (102 pg mL-1). The developed reusable immunosensor has demonstrated high-performance characteristics by requiring less than 50 µL of sample and providing analysis in less than 25 min. This new method could impact the development of point-of-care devices for biomedical applications.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Técnicas Biosensibles
/
Microfluídica
Idioma:
En
Revista:
Anal Methods
Año:
2023
Tipo del documento:
Article
País de afiliación:
Japón