Characterization of the immune infiltrate in mouse tissue by multiplex immunofluorescence.
Methods Cell Biol
; 174: 43-53, 2023.
Article
en En
| MEDLINE
| ID: mdl-36710050
ABSTRACT
Multiplexed immunofluorescence imaging of formalin-fixed, paraffin-embedded (FFPE) specimens mounted on glass slides allow the identification of multiple cell phenotypes while retaining spatial and morphological context. Multiplex immunofluorescence protocols have already been developed and validated for mouse tissues. Immunophenotyping analysis reliably depicts the immune landscape of cancer tissues that has been demonstrated to influence cancer development and progression as well as to have an impact on therapy responsiveness and resistance. Here, we describe a method for multiplexed fluorescence image analysis, enabling analysis of mouse cancer morphology and cell phenotypes in FFPE sections.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Neoplasias
Tipo de estudio:
Guideline
Límite:
Animals
Idioma:
En
Revista:
Methods Cell Biol
Año:
2023
Tipo del documento:
Article
País de afiliación:
Reino Unido