Gamma Knife Radiosurgery Modulates micro-RNA Levels in Patients with Brain Metastasis.
Curr Radiopharm
; 16(3): 204-213, 2023 Jun 05.
Article
en En
| MEDLINE
| ID: mdl-36733208
ABSTRACT
BACKGROUND:
The relation between micro-RNA (miRNA) modulation and immune cell activity in high-dose radiation settings is not clearly understood.OBJECTIVE:
To investigate the role of stereotactic radiosurgery (SRS) in (i) the regulation of tumorsuppressor and oncogenic miRNAs as well as (ii) its effect on specific immune cell subsets in patients with metastatic brain tumors (MBT).METHODS:
9 MBT patients who underwent gamma knife-based stereotactic radiosurgery (GKRS) and 8 healthy individuals were included. Serum samples were isolated at three-time intervals (before GKRS, 1 hour, and 1-month post-GKRS). Expressions of tumor-suppressor (miR-124) and oncogenic (miR-21, miR-181a, miR-23a, miR-125b, and miR-17) miRNAs were quantified by qPCR. The lymphocytic frequency (CD3+, CD4+, CD8+, CD56+, CD19+, and CD16+) was investigated by means of flow cytometry.RESULTS:
The median age was 64 years (range 50-73 years). The median prescription dose was 20Gy (range 16Gy-24Gy), all delivered in a single fraction. The median overall survival and progression- free survival were 7.8 months (range 1.7-14.9 months) and 6.7 months (range 1.1-11.5 months), respectively. Compared to healthy controls, baseline levels of oncogenic miRNAs were significantly higher, while tumor-suppressing miRNA levels remained markedly lower in MBT patients prior to GKRS. Following GKRS, there was a reduction in the expression of miR-21, miR-17, and miR-181a; simultaneously, increased expression increased of miR-124 was observed. No significant difference in immune cell subsets was noted post GKRSIn a similar fashion. We noted no correlation between patient characteristics, radiosurgery data, miRNA expression, and immune cell frequency.CONCLUSION:
For this specific population with MBT disease, our data suggest that stereotactic radiosurgery may modulate the expression of circulating tumor-suppressor and oncogenic miRNAs, ultimately enhancing key anti-tumoral responses. Further evaluation with larger cohorts is warranted.Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Neoplasias Encefálicas
/
Radiocirugia
/
MicroARNs
Tipo de estudio:
Observational_studies
/
Prognostic_studies
Límite:
Humans
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Middle aged
Idioma:
En
Revista:
Curr Radiopharm
Año:
2023
Tipo del documento:
Article
País de afiliación:
Turquía