Novel ultrasensitive Raman assay method based on enzyme mimetics for ultra trace of H2O2.
Spectrochim Acta A Mol Biomol Spectrosc
; 293: 122456, 2023 May 15.
Article
en En
| MEDLINE
| ID: mdl-36773420
ABSTRACT
Enzyme mimetics have been widely applied on H2O2 assay, but it is still challenging and interesting to realize the sensitive detection for ultra-trace H2O2. Here, an ultrasensitive Raman assay method based on novel WO3@IP6-Fe3+ enzyme mimetics with peroxidase-like activity was established. WO3 microspheres (MSs) were found to have weak peroxidase-like activity, and the combination of IP6-Fe3+ and WO3 can produce stronger activity. WO3@IP6-Fe3+ MSs showed polyhedron-like structure, uniform size, and smooth surface. Although WO3@IP6-Fe3+ enzyme mimetics have low catalytic efficiency and high absorbance background, the proposed Raman method can bypass the above problems. In Raman method, high concentration of WO3@IP6-Fe3+ can be used to overcome low catalytic efficiency without high absorbance background. Moreover, 3,3',5,5'-tetramethylbenzidine oxide has prominent characteristic Raman peak at 1608 cm-1, greatly improving the sensitivity and eliminating interference of impurities. Due to the high sensitivity and low background, Raman assay showed the ultra-low limit of detection (5.49 × 10-15 M), which was 4-7 orders of magnitude lower than other detection methods. The ultrasensitive Raman assay not only provided the possibility for the enzyme mimetics-based detection of ultra-trace H2O2, but also enable the enzyme mimetics with low activity to be applied.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Peroxidasa
/
Peróxido de Hidrógeno
Idioma:
En
Revista:
Spectrochim Acta A Mol Biomol Spectrosc
Asunto de la revista:
BIOLOGIA MOLECULAR
Año:
2023
Tipo del documento:
Article
País de afiliación:
China