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Aggrecan accumulates at sites of increased pulmonary arterial pressure in idiopathic pulmonary arterial hypertension.
van der Have, Oscar; Mead, Timothy J; Westöö, Christian; Peruzzi, Niccolò; Mutgan, Ayse C; Norvik, Christian; Bech, Martin; Struglics, André; Hoetzenecker, Konrad; Brunnström, Hans; Westergren-Thorsson, Gunilla; Kwapiszewska, Grazyna; Apte, Suneel S; Tran-Lundmark, Karin.
Afiliación
  • van der Have O; Department of Experimental Medical Science, Faculty of Medicine Lund University Lund Sweden.
  • Mead TJ; Department of Biomedical Engineering Cleveland Clinic Lerner Research Institute Cleveland Ohio USA.
  • Westöö C; Department of Experimental Medical Science, Faculty of Medicine Lund University Lund Sweden.
  • Peruzzi N; Department of Experimental Medical Science, Faculty of Medicine Lund University Lund Sweden.
  • Mutgan AC; Department of Medical Radiation Physics, Clinical Sciences Lund Lund University Lund Sweden.
  • Norvik C; Ludwig Boltzmann Institute for Lung Vascular Research Graz Austria.
  • Bech M; Division of Physiology, Otto Loewi Research Center Medical University Graz Graz Austria.
  • Struglics A; Department of Experimental Medical Science, Faculty of Medicine Lund University Lund Sweden.
  • Hoetzenecker K; Department of Medical Radiation Physics, Clinical Sciences Lund Lund University Lund Sweden.
  • Brunnström H; Department of Clinical Sciences Lund, Orthopaedics, Faculty of Medicine Lund University Lund Sweden.
  • Westergren-Thorsson G; Department of Thoracic Surgery Medical University of Vienna Vienna Austria.
  • Kwapiszewska G; Department of Clinical Sciences Lund, Division of Pathology, Faculty of Medicine Lund University Lund Sweden.
  • Apte SS; Department of Genetics and Pathology Division of Laboratory Medicine Lund Sweden.
  • Tran-Lundmark K; Department of Experimental Medical Science, Faculty of Medicine Lund University Lund Sweden.
Pulm Circ ; 13(1): e12200, 2023 Jan.
Article en En | MEDLINE | ID: mdl-36824691
ABSTRACT
Expansion of extracellular matrix occurs in all stages of pulmonary angiopathy associated with pulmonary arterial hypertension (PAH). In systemic arteries, dysregulation and accumulation of the large chondroitin-sulfate proteoglycan aggrecan is associated with swelling and disruption of vessel wall homeostasis. Whether aggrecan is present in pulmonary arteries, and its potential roles in PAH, has not been thoroughly investigated. Here, lung tissue from 11 patients with idiopathic PAH was imaged using synchrotron radiation phase-contrast microcomputed tomography (TOMCAT beamline, Swiss Light Source). Immunohistochemistry for aggrecan core protein in subsequently sectioned lung tissue demonstrated accumulation in PAH compared with failed donor lung controls. RNAscope in situ hybridization indicated ACAN expression in vascular endothelium and smooth muscle cells. Based on qualitative histological analysis, aggrecan localizes to cellular, rather than fibrotic or collagenous, lesions. Interestingly, ADAMTS15, a potential aggrecanase, was upregulated in pulmonary arteries in PAH. Aligning traditional histological analysis with three-dimensional renderings of pulmonary arteries from synchrotron imaging identified aggrecan in lumen-reducing lesions containing loose, cell-rich connective tissue, at sites of intrapulmonary bronchopulmonary shunting, and at sites of presumed elevated pulmonary blood pressure. Our findings suggest that ACAN expression may be an early response to injury in pulmonary angiopathy and supports recent work showing that dysregulation of aggrecan turnover is a hallmark of arterial adaptations to altered hemodynamics. Whether cause or effect, aggrecan and aggrecanase regulation in PAH are potential therapeutic targets.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Tipo de estudio: Prognostic_studies / Qualitative_research Idioma: En Revista: Pulm Circ Año: 2023 Tipo del documento: Article

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Tipo de estudio: Prognostic_studies / Qualitative_research Idioma: En Revista: Pulm Circ Año: 2023 Tipo del documento: Article
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