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Andrographolide suppresses aerobic glycolysis and induces apoptotic cell death by inhibiting pyruvate dehydrogenase kinase 1 expression.
Yang, Eun-Sun; Do, Yunju; Cheon, Se-Yun; Kim, Bosung; Ling, Jin; Cho, Min Kyoung; Kim, Taekyung; Bae, Sung-Jin; Ha, Ki-Tae.
Afiliación
  • Yang ES; Korean Medical Research Center for Healthy Aging, Pusan National University, Yangsan, Gyeongsangnam 50612, Republic of Korea.
  • Do Y; Department of Korean Medical Science, School of Korean Medicine, Pusan National University, Yangsan, Gyeongsangnam 50612, Republic of Korea.
  • Cheon SY; Korean Medical Research Center for Healthy Aging, Pusan National University, Yangsan, Gyeongsangnam 50612, Republic of Korea.
  • Kim B; Department of Korean Medical Science, School of Korean Medicine, Pusan National University, Yangsan, Gyeongsangnam 50612, Republic of Korea.
  • Ling J; Department of Korean Medical Science, School of Korean Medicine, Pusan National University, Yangsan, Gyeongsangnam 50612, Republic of Korea.
  • Cho MK; Korean Medical Research Center for Healthy Aging, Pusan National University, Yangsan, Gyeongsangnam 50612, Republic of Korea.
  • Kim T; Department of Biology Education, Pusan National University, Busan 46241, Republic of Korea.
  • Bae SJ; Department of Molecular Biology and Immunology, Kosin University College of Medicine, Busan 49267, Republic of Korea.
  • Ha KT; Korean Medical Research Center for Healthy Aging, Pusan National University, Yangsan, Gyeongsangnam 50612, Republic of Korea.
Oncol Rep ; 49(4)2023 04.
Article en En | MEDLINE | ID: mdl-36825595
ABSTRACT
Metabolic disorder is a major characteristic of cancer cells, and controlling genes involved in metabolic shifts can be an effective strategy for cancer treatment. Andrographolide (AG), a diterpenoid lactone, is widely recognized as a natural anticancer drug due to its ability to inhibit cancer growth. The present study aimed to investigate the mechanism underlying the mitochondrial­mediated anticancer effect of AG by inhibiting pyruvate dehydrogenase kinase 1 (PDK1) expression in lung cancer cells. Cells were treated with AG and PDK1 mRNA and protein expression was determined using reverse transcription­quantitative PCR and western blotting, respectively. As a result, AG significantly inhibited the viability of human lung cancer cells and suppressed aerobic glycolysis by decreasing lactate generation. AG further decreased the PDK1 protein and mRNA levels in a dose­dependent manner. AG­induced cell death was assessed by flow cytometry and fluorescence microscopy. AG induced apoptotic cell death that was associated with the cleavage of poly (ADP ribose) polymerase, activation of caspase­3, and mitochondrial damage, which was associated with an increase in reactive oxygen species and loss of mitochondrial membrane potential. AG­induced cell death was partially suppressed via PDK1 overexpression in lung cancer cells. Therefore, the anticancer effects of AG on human lung cancer cells may negatively regulate the expression of PDK1.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Contexto en salud: 6_ODS3_enfermedades_notrasmisibles Problema de salud: 6_other_respiratory_diseases / 6_trachea_bronchus_lung_cancer Asunto principal: Diterpenos / Neoplasias Pulmonares Límite: Humans Idioma: En Revista: Oncol Rep Asunto de la revista: NEOPLASIAS Año: 2023 Tipo del documento: Article

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Contexto en salud: 6_ODS3_enfermedades_notrasmisibles Problema de salud: 6_other_respiratory_diseases / 6_trachea_bronchus_lung_cancer Asunto principal: Diterpenos / Neoplasias Pulmonares Límite: Humans Idioma: En Revista: Oncol Rep Asunto de la revista: NEOPLASIAS Año: 2023 Tipo del documento: Article
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