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Liquid crystal-based sensor for real-time detection of paraoxon pesticides based on acetylcholinesterase enzyme inhibition.
Duong, Duong Song Thai; Jang, Chang-Hyun.
Afiliación
  • Duong DST; Department of Chemistry, Gachon University, San 65, Bokjeong-Dong, Sujeong-Gu, Gyeonggi-Do, 461-701, Seongnam-City, South Korea.
  • Jang CH; Department of Chemistry, Gachon University, San 65, Bokjeong-Dong, Sujeong-Gu, Gyeonggi-Do, 461-701, Seongnam-City, South Korea. chjang4u@gachon.ac.kr.
Mikrochim Acta ; 190(4): 122, 2023 03 09.
Article en En | MEDLINE | ID: mdl-36890280
A liquid crystal-based assay (LC) was developed to monitor paraoxon by incorporating a Cu2+ -coated substrate and the inhibitory effect of paraoxon with acetylcholinesterase (AChE). We observed that thiocholine (TCh), a hydrolysate of AChE and acetylthiocholine (ATCh), interfered with the alignment of 5CB films through a reaction between Cu2+ ions and the thiol moiety of TCh. The catalytic activity of AChE was inhibited in the presence of paraoxon due to the irreversible interaction between TCh and paraoxon; consequently, no TCh molecule was available to interact with Cu2+ on the surface. This resulted in a homeotropic alignment of the liquid crystal. The proposed sensor platform sensitively quantified paraoxon with a detection limit of 2.20 ± 0.11 (n = 3) nM within a range of 6 to 500 nM. The specificity and reliability of the assay were verified by measuring paraoxon in the presence of various suspected interfering substances and spiked samples. As a result, the sensor based on LC can potentially be used as a screening tool for accurate evaluation of paraoxon and other organophosphorus compounds.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Plaguicidas / Cristales Líquidos Tipo de estudio: Diagnostic_studies Idioma: En Revista: Mikrochim Acta Año: 2023 Tipo del documento: Article País de afiliación: Corea del Sur

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Plaguicidas / Cristales Líquidos Tipo de estudio: Diagnostic_studies Idioma: En Revista: Mikrochim Acta Año: 2023 Tipo del documento: Article País de afiliación: Corea del Sur
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