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High-performance glass filters for capturing and culturing circulating tumor cells and cancer-associated fibroblasts.
Tanaka, Hiromasa; Iwata, Daijiro; Shibata, Yuki; Hase, Tetsunari; Onoshima, Daisuke; Yogo, Naoyuki; Shibata, Hirofumi; Sato, Mitsuo; Ishikawa, Kenji; Nagasawa, Ikuo; Hasegawa, Yoshinori; Ishii, Makoto; Baba, Yoshinobu; Hori, Masaru.
Afiliación
  • Tanaka H; Center for Low-Temperature Plasma Sciences, Nagoya University, Furo-cho, Chikusa-ku, Nagoya, 464-8601, Japan. htanaka@plasma.engg.nagoya-u.ac.jp.
  • Iwata D; Innovative Technology Laboratories, AGC Inc., 1-1 Suehirocho Tsurumi-ku, Yokohama, 230-0045, Japan.
  • Shibata Y; Center for Low-Temperature Plasma Sciences, Nagoya University, Furo-cho, Chikusa-ku, Nagoya, 464-8601, Japan.
  • Hase T; Department of Respiratory Medicine, Nagoya University Graduate School of Medicine, 65 Tsurumai-cho, Showa-ku, Nagoya, 466-8550, Japan.
  • Onoshima D; Institute of Nano-Life-Systems, Institute of Innovation for Future Society, Nagoya University, Nagoya, 464-8601, Japan.
  • Yogo N; Department of Respiratory Medicine, Nagoya University Graduate School of Medicine, 65 Tsurumai-cho, Showa-ku, Nagoya, 466-8550, Japan.
  • Shibata H; Department of Respiratory Medicine, Nagoya University Graduate School of Medicine, 65 Tsurumai-cho, Showa-ku, Nagoya, 466-8550, Japan.
  • Sato M; Division of Host Defense Sciences, Department of Integrated Health Sciences, Nagoya University Graduate School of Medicine, Daikominami 1-1-20, Higashi-ku, Nagoya, Japan.
  • Ishikawa K; Center for Low-Temperature Plasma Sciences, Nagoya University, Furo-cho, Chikusa-ku, Nagoya, 464-8601, Japan.
  • Nagasawa I; Innovative Technology Laboratories, AGC Inc., 1-1 Suehirocho Tsurumi-ku, Yokohama, 230-0045, Japan.
  • Hasegawa Y; Department of Respiratory Medicine, Nagoya University Graduate School of Medicine, 65 Tsurumai-cho, Showa-ku, Nagoya, 466-8550, Japan.
  • Ishii M; National Hospital Organization, Nagoya Medical Center, 4-1-1, Sannomaru, Naka-ku, Nagoya, 460-0001, Japan.
  • Baba Y; Department of Respiratory Medicine, Nagoya University Graduate School of Medicine, 65 Tsurumai-cho, Showa-ku, Nagoya, 466-8550, Japan.
  • Hori M; Institute of Nano-Life-Systems, Institute of Innovation for Future Society, Nagoya University, Nagoya, 464-8601, Japan.
Sci Rep ; 13(1): 4130, 2023 03 13.
Article en En | MEDLINE | ID: mdl-36914725
Various liquid biopsy methods have been developed for the non-invasive and early detection of diseases. In particular, the detection of circulating tumor cells (CTCs) and cancer-associated fibroblasts (CAFs) in blood has been receiving a great deal of attention. We have been developing systems and materials to facilitate such liquid biopsies. In this study, we further developed glass filters (with various patterns of holes, pitches, and non-adhesive coating) that can capture CTCs, but not white blood cells. We optimized the glass filters to capture CTCs, and demonstrated that they could be used to detect CTCs from lung cancer patients. We also used the optimized glass filters for detecting CAFs. Additionally, we further developed a system for visualizing the captured cells on the glass filters. Finally, we demonstrated that we could directly culture the captured cells on the glass filters. Based on these results, our high-performance glass filters appear to be useful for capturing and culturing CTCs and CAFs for further examinations.
Asunto(s)

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Fibroblastos Asociados al Cáncer / Neoplasias Pulmonares / Células Neoplásicas Circulantes Tipo de estudio: Diagnostic_studies / Risk_factors_studies / Screening_studies Límite: Humans Idioma: En Revista: Sci Rep Año: 2023 Tipo del documento: Article País de afiliación: Japón

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Fibroblastos Asociados al Cáncer / Neoplasias Pulmonares / Células Neoplásicas Circulantes Tipo de estudio: Diagnostic_studies / Risk_factors_studies / Screening_studies Límite: Humans Idioma: En Revista: Sci Rep Año: 2023 Tipo del documento: Article País de afiliación: Japón
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