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A retrospective cohort study of canid herpesvirus-1 seroprevalence and epidemiology in kennel dogs in Croatia.
Gracin, Koraljka; Mauric Maljkovic, Maja; Barbic, Ljubo; Staresina, Vilim; Lojkic, Martina; Stevanovic, Vladimir.
Afiliación
  • Gracin K; LunimirVet, Private Veterinary Clinic, Kraljeviceva 20, Zagreb, Croatia.
  • Mauric Maljkovic M; Department of Animal Breeding and Livestock Production, Faculty of Veterinary Medicine, University of Zagreb, Heinzelova 55, Zagreb, Croatia.
  • Barbic L; Department of Microbiology and Infectious Diseases with Clinic, Faculty of Veterinary Medicine, University of Zagreb, Heinzelova 55, Zagreb, Croatia.
  • Staresina V; Department of Microbiology and Infectious Diseases with Clinic, Faculty of Veterinary Medicine, University of Zagreb, Heinzelova 55, Zagreb, Croatia.
  • Lojkic M; Reproduction and Obstetrics Clinic, Faculty of Veterinary Medicine, University of Zagreb, Heinzelova 55, Zagreb, Croatia.
  • Stevanovic V; Department of Microbiology and Infectious Diseases with Clinic, Faculty of Veterinary Medicine, University of Zagreb, Heinzelova 55, Zagreb, Croatia. Electronic address: vstevanovic@vef.unizg.hr.
Res Vet Sci ; 158: 117-123, 2023 May.
Article en En | MEDLINE | ID: mdl-37003119
Canid alphaherpesvirus-1 (CaHV-1) is an endemic pathogen of dogs worldwide. CaHV-1 is often related to abortions, neonatal mortality, and the death of puppies. Since the first description of the virus in 1965, there has been no generally accepted method for diagnosing CaHV-1. Many authors used the virus neutralisation test (VNT) as a 'reference standard' due to its high specificity. Nasal, vaginal, preputial swabs and serum samples for this study were collected in the population of kennel dogs in Croatia. To determine the optimal VNT protocol, three modifications of the VNT were compared. These modifications were: VNT using native serum samples, VNT using thermally inactivated serum samples, and VNT using thermally inactivated serum samples with the addition of the complement. The correlation between the results of the VNT methods was significant (P < 0.001). Of all three modifications of VNT, the one using native serum samples was the one that increased VNT sensitivity. The overall seroprevalence of CaHV-1 was 32.02%. The PCR did not confirm the CaHV-1 presence in collected swabs. According to analysed anamnestic data, significant risk factors for CaHV-1 infection were: kennel size, attendance at the dog shows, hunt trials, kennel disinfection protocols, and mating. The oestrus cycle had no significant effect on seropositivity. The study results indicate that CaHV-1 spreads horizontally between dams living in kennels and in males during mating. Although there was no correlation between seropositivity and a history of reproductive disorders, significantly more stillborn puppies were recorded in seronegative dames (P < 0.01).
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Herpesvirus Cánido 1 / Infecciones por Herpesviridae / Enfermedades de los Perros Tipo de estudio: Etiology_studies / Guideline / Observational_studies / Risk_factors_studies / Screening_studies Límite: Animals / Pregnancy País/Región como asunto: Europa Idioma: En Revista: Res Vet Sci Año: 2023 Tipo del documento: Article País de afiliación: Croacia

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Herpesvirus Cánido 1 / Infecciones por Herpesviridae / Enfermedades de los Perros Tipo de estudio: Etiology_studies / Guideline / Observational_studies / Risk_factors_studies / Screening_studies Límite: Animals / Pregnancy País/Región como asunto: Europa Idioma: En Revista: Res Vet Sci Año: 2023 Tipo del documento: Article País de afiliación: Croacia
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