smalldisco, a pipeline for siRNA discovery and 3' tail identification.
G3 (Bethesda)
; 13(6)2023 06 01.
Article
en En
| MEDLINE
| ID: mdl-37094819
ABSTRACT
Capturing and sequencing small RNAs is standard practice; however, identification of a group of these small RNAs-small interfering RNAs (siRNAs)-has been more difficult. We present smalldisco, a command-line tool for small interfering RNA discovery and annotation from small RNA-seq datasets. smalldisco can distinguish short reads that map antisense to an annotated genomic feature (e.g. exons or mRNAs), annotate these siRNAs, and quantify their abundance. smalldisco also uses the program Tailor to quantify 3' nontemplated nucleotides of siRNAs or any small RNA species. smalldisco and supporting documentation are available for download from GitHub (https//github.com/ianvcaldas/smalldisco) and archived in Zenodo (https//doi.org/10.5281/zenodo.7799621).
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Genoma
/
Genómica
Tipo de estudio:
Diagnostic_studies
Idioma:
En
Revista:
G3 (Bethesda)
Año:
2023
Tipo del documento:
Article
País de afiliación:
Estados Unidos