Your browser doesn't support javascript.
loading
Zona pellucida family genes in Chinese pond turtle: identification, expression profiles, and role in the spermatozoa acrosome reaction†.
Dong, Jinxiu; Pei, Kejiao; Xu, Wannan; Gong, Mengmeng; Zhu, Wenrui; Liu, Siqi; Tang, Min; Liu, Jianjun; Xia, Xingquan; Bu, Xinjiang; Nie, Liuwang.
Afiliación
  • Dong J; Provincial Key Lab of the Conservation and Exploitation Research of Biological Resources in Anhui, College of Life Sciences, Anhui Normal University, Wuhu, Anhui, China.
  • Pei K; Provincial Key Lab of the Conservation and Exploitation Research of Biological Resources in Anhui, College of Life Sciences, Anhui Normal University, Wuhu, Anhui, China.
  • Xu W; Provincial Key Lab of the Conservation and Exploitation Research of Biological Resources in Anhui, College of Life Sciences, Anhui Normal University, Wuhu, Anhui, China.
  • Gong M; Provincial Key Lab of the Conservation and Exploitation Research of Biological Resources in Anhui, College of Life Sciences, Anhui Normal University, Wuhu, Anhui, China.
  • Zhu W; Provincial Key Lab of the Conservation and Exploitation Research of Biological Resources in Anhui, College of Life Sciences, Anhui Normal University, Wuhu, Anhui, China.
  • Liu S; Provincial Key Lab of the Conservation and Exploitation Research of Biological Resources in Anhui, College of Life Sciences, Anhui Normal University, Wuhu, Anhui, China.
  • Tang M; Provincial Key Lab of the Conservation and Exploitation Research of Biological Resources in Anhui, College of Life Sciences, Anhui Normal University, Wuhu, Anhui, China.
  • Liu J; Provincial Key Lab of the Conservation and Exploitation Research of Biological Resources in Anhui, College of Life Sciences, Anhui Normal University, Wuhu, Anhui, China.
  • Xia X; Provincial Key Lab of the Conservation and Exploitation Research of Biological Resources in Anhui, College of Life Sciences, Anhui Normal University, Wuhu, Anhui, China.
  • Bu X; Provincial Key Lab of the Conservation and Exploitation Research of Biological Resources in Anhui, College of Life Sciences, Anhui Normal University, Wuhu, Anhui, China.
  • Nie L; Provincial Key Lab of the Conservation and Exploitation Research of Biological Resources in Anhui, College of Life Sciences, Anhui Normal University, Wuhu, Anhui, China.
Biol Reprod ; 109(1): 97-106, 2023 07 11.
Article en En | MEDLINE | ID: mdl-37140246
ABSTRACT
The zona pellucida (ZP) is an extracellular matrix that surrounds all vertebrate eggs, and it is involved in fertilization and species-specific recognition. Numerous in-depth studies of the ZP proteins of mammals, birds, amphibians, and fishes have been conducted, but systematic investigation of the ZP family genes and their role during fertilization in reptiles has not been reported to date. In this study, we identified six turtle ZP (Tu-ZP) gene subfamilies (Tu-ZP1, Tu-ZP2, Tu-ZP3, Tu-ZP4, Tu-ZPD, and Tu-ZPAX) based on whole genome sequence data from Mauremys reevesii. We found that Tu-ZP4 had large segmental duplication and was distributed on three chromosomes, and we also detected gene duplication in the other Tu-ZP genes. To evaluate the role of Tu-ZP proteins in sperm-egg binding, we assessed the expression pattern of these Tu-ZP proteins and their ability to induce the spermatozoa acrosome reaction in M. reevesii. In conclusion, this is the first report of the existence of gene duplication of Tu-ZP genes and that Tu-ZP2, Tu-ZP3, and Tu-ZPD can induce acrosome exocytosis of spermatogenesis in the reptile.
Asunto(s)
Palabras clave
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Tortugas / Reacción Acrosómica Tipo de estudio: Diagnostic_studies Límite: Animals Idioma: En Revista: Biol Reprod Año: 2023 Tipo del documento: Article País de afiliación: China
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Tortugas / Reacción Acrosómica Tipo de estudio: Diagnostic_studies Límite: Animals Idioma: En Revista: Biol Reprod Año: 2023 Tipo del documento: Article País de afiliación: China