Whole-mount immunofluorescence staining of blood and lymphatic vessels in murine small intestine.
STAR Protoc
; 4(2): 102310, 2023 May 13.
Article
en En
| MEDLINE
| ID: mdl-37182207
ABSTRACT
The small intestine is an excellent model for studying changes in vasculature in response to different diseases or gene deletions. Here, we present a protocol for whole-mount immunofluorescence staining of blood and lymphatic vessels in the adult mouse small intestine. We describe the steps for perfusion fixation, tissue sample preparation, immunofluorescence staining, and whole-mount preparation of stained samples. Our protocol will enable researchers to visualize and analyze the intricate network of vessels in the small intestine. For complete details on the use and execution of this protocol, please refer to Karaman et al. (2022).1.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Idioma:
En
Revista:
STAR Protoc
Año:
2023
Tipo del documento:
Article
País de afiliación:
Finlandia