In vitro effects of monophosphoryl lipid A and Poly I:C combination on equine cells.
J Vet Sci
; 24(3): e37, 2023 May.
Article
en En
| MEDLINE
| ID: mdl-37271505
ABSTRACT
BACKGROUND:
Toll-like receptor (TLR) agonists have been used as adjuvants to modulate immune responses in both animals and humans.OBJECTIVES:
The objective of this study was to evaluate the combined effects of the TLR 4 agonist monophosphoryl lipid A (MPL) and the TLR 3 agonist polyinosinicpolycytidylic acid (Poly IC) on equine peripheral blood mononuclear cells (PBMCs), monocyte-derived dendritic cells (MoDCs), and bone marrow-derived mesenchymal stromal cells (BM-MSCs).METHODS:
The PBMCs, MoDCs, and BM-MSCs collected from three mixed breed horses were treated with MPL, Poly IC, and their combination. The mRNA expression of interferon gamma (IFN-γ), interleukin (IL)-1ß, IL-4, IL-6, IL-8, IL-12p40, tumor necrosis factor alpha (TNF-α), vascular endothelial growth factor (VEGF), and monocyte chemoattractant protein-1 (MCP-1) was determined using real-time polymerase chain reaction.RESULTS:
The combination of MPL and Poly IC significantly upregulated immunomodulatory responses in equine cells/ without cytotoxicity. The combination induced greater mRNA expression of pro-inflammatory cytokines IFN-γ and IL-6 than MPL or Poly IC stimulation alone in PBMCs. In addition, the combination induced significantly higher mRNA expression of IL-1ß, IL-6, and IL-12p40 in MoDCs, and IL-8, MCP-1, and VEGF in BM-MSCs compared to stimulation with a single TLR agonist.CONCLUSIONS:
The combination of MPL and Poly IC can be used as a potential adjuvant candidate for vaccines to aid in preventing infectious diseases in horses.Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Leucocitos Mononucleares
/
Factor A de Crecimiento Endotelial Vascular
Límite:
Animals
/
Humans
Idioma:
En
Revista:
J Vet Sci
Asunto de la revista:
MEDICINA VETERINARIA
Año:
2023
Tipo del documento:
Article