Generation of human-induced pluripotent-stem-cell-derived cortical neurons for high-throughput imaging of neurite morphology and neuron maturation.

Wali, Gautam; Li, Yan; Abu-Bonsrah, Dad; Kirik, Deniz; Parish, Clare L; Sue, Carolyn M

Wali, Gautam; Li, Yan; Abu-Bonsrah, Dad; Kirik, Deniz; Parish, Clare L; Sue, Carolyn M.

Afiliación

Wali G; Neuroscience Research Australia and University of New South Wales, Sydney, NSW 2031, Australia; Kolling Institute for Medical Research and Department of Medicine, University of Sydney, Sydney, NSW 2065, Australia; Northern Clinical School, Sydney Medical School, University of Sydney, Sydney, NSW 206
Li Y; Neuroscience Research Australia and University of New South Wales, Sydney, NSW 2031, Australia; Kolling Institute for Medical Research and Department of Medicine, University of Sydney, Sydney, NSW 2065, Australia; Northern Clinical School, Sydney Medical School, University of Sydney, Sydney, NSW 206
Abu-Bonsrah D; The Florey Institute of Neuroscience and Mental Health, The University of Melbourne, Parkville, VIC 3052, Australia; Department of Paediatrics, The University of Melbourne, Parkville, VIC 3052, Australia; Aligning Science Across Parkinson's (ASAP) Collaborative Research Network, Chevy Chase, MD 2081
Kirik D; BRAINS Unit, BMC D11, Lund University, 22184 Lund, Sweden; Honorary Professorship at School of Medical Sciences, Faculty of Medicine and Health, The University of Sydney, Sydney, NSW 2006, Australia; Aligning Science Across Parkinson's (ASAP) Collaborative Research Network, Chevy Chase, MD 20815, US
Parish CL; The Florey Institute of Neuroscience and Mental Health, The University of Melbourne, Parkville, VIC 3052, Australia; Aligning Science Across Parkinson's (ASAP) Collaborative Research Network, Chevy Chase, MD 20815, USA.
Sue CM; Neuroscience Research Australia and University of New South Wales, Sydney, NSW 2031, Australia; Kolling Institute for Medical Research and Department of Medicine, University of Sydney, Sydney, NSW 2065, Australia; Northern Clinical School, Sydney Medical School, University of Sydney, Sydney, NSW 206

STAR Protoc ; 4(2): 102325, 2023 Jun 09.

Article en En | MEDLINE | ID: mdl-37300830

ABSTRACT

ABSTRACT

High-throughput imaging allows in vitro assessment of neuron morphology for screening populations under developmental, homeostatic, and/or disease conditions. Here, we present a protocol to differentiate cryopreserved human cortical neuronal progenitors into mature cortical neurons for high-throughput imaging analysis. We describe the use of a notch signaling inhibitor to generate homogeneous neuronal populations at densities amenable to individual neurite identification. We detail neurite morphology assessment via measuring multiple parameters including neurite length, branches, roots, segments and extremities, and neuron maturation.

Palabras clave

Cell Biology; Cell Differentiation; Cell culture; Microscopy; Neuroscience; Stem Cells

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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: STAR Protoc Año: 2023 Tipo del documento: Article