Effect of postharvest nicotinamide treatment on NAD+ metabolism and redox status in strawberry fruit during storage.
Phytochemistry
; 213: 113766, 2023 Sep.
Article
en En
| MEDLINE
| ID: mdl-37343736
ABSTRACT
The increased activity of PARP enzymes is associated with a deficiency of NAD+, as well as with a loss of NADPH and ATP, and consequent deterioration of the redox state in fruits. In this study, we checked whether treatment with nicotinamide (NAM) would affect PARP-1 expression and NAD+ metabolism in strawberry fruit during storage. For this purpose, strawberry fruits were treated with 10 mM NAM and co-treated with NAM and UV-C, and then stored for 5 days at 4 °C. Research showed that nicotinamide contributes to reducing oxidative stress level by reducing PARP-1 mRNA gene expression and the protein level resulting in higher NAD+ availability, as well as improving energy metabolism and NADPH levels in fruits, regardless of whether they are exposed to UV-C. The above effects cause fruits treated with nicotinamide to be characterised by higher anti-radical activity, and a lower level of reactive oxygen species in the tissue.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Niacinamida
/
Fragaria
/
Almacenamiento de Alimentos
/
Frutas
Idioma:
En
Revista:
Phytochemistry
Año:
2023
Tipo del documento:
Article