The immune response to arterial damage in a mouse model of intermittent hypoxia: a transcriptomics analysis.
Sleep Breath
; 27(6): 2397-2406, 2023 12.
Article
en En
| MEDLINE
| ID: mdl-37391539
ABSTRACT
PURPOSE:
Mice can develop arterial damage and even atherosclerosis under intermittent hypoxia (IH); however, the specific mechanism of arterial damage induced by IH remains unclear. Hence, this research aimed to illustrate the underlying mechanism linking IH to arterial injury. MATERIALS ANDMETHODS:
The differential gene expression of the thoracic aorta under normoxia or IH mice was analyzed utilizing RNA sequencing. Furthermore, GO, KEGG pathway, and CIBERSORT analyses were carried out. For verification of the expression of candidate genes affected by IH, quantitative RT-qPCR (qRT-PCR) was conducted. Immunohistochemical (IHC) staining revealed immune cell infiltration in the thoracic aorta.RESULTS:
The thickness of the intima-media of the mouse aorta was increased, and the fiber structure was disordered under IH. Transcriptomics analysis showed that in the aorta, 1137 upregulated genes and 707 downregulated genes were affected by IH, significantly related to the activation of the immune system and cell adhesion. Furthermore, B cell infiltration around the aorta was observed under IH.CONCLUSIONS:
IH might lead to structural changes in the aorta by activating the immune response and enhancing cell adhesion.Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Transcriptoma
/
Hipoxia
Límite:
Animals
Idioma:
En
Revista:
Sleep Breath
Asunto de la revista:
NEUROLOGIA
/
OTORRINOLARINGOLOGIA
Año:
2023
Tipo del documento:
Article
País de afiliación:
China