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Lectin-Seq: A method to profile lectin-microbe interactions in native communities.
McPherson, Robert L; Isabella, Christine R; Walker, Rebecca L; Sergio, Dallis; Bae, Sunhee; Gaca, Tony; Raman, Smrithi; Nguyen, Le Thanh Tu; Wesener, Darryl A; Halim, Melanie; Wuo, Michael G; Dugan, Amanda; Kerby, Robert; Ghosh, Soumi; Rey, Federico E; Dhennezel, Catherine; Pishchany, Gleb; Lensch, Valerie; Vlamakis, Hera; Alm, Eric J; Xavier, Ramnik J; Kiessling, Laura L.
Afiliación
  • McPherson RL; Department of Chemistry, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
  • Isabella CR; Center for Microbiome Informatics and Therapeutics, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
  • Walker RL; Department of Chemistry, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
  • Sergio D; Center for Microbiome Informatics and Therapeutics, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
  • Bae S; The Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA.
  • Gaca T; The Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA.
  • Raman S; Department of Chemistry, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
  • Nguyen LTT; The Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA.
  • Wesener DA; Department of Chemistry, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
  • Halim M; Center for Microbiome Informatics and Therapeutics, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
  • Wuo MG; Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
  • Dugan A; Edison Family Center for Genome Sciences & Systems Biology, Washington University School of Medicine, Saint Louis, MO 63110, USA.
  • Kerby R; Center for Gut Microbiome and Nutrition Research, Washington University School of Medicine, Saint Louis, MO 63110, USA.
  • Ghosh S; Department of Chemistry, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
  • Rey FE; Center for Microbiome Informatics and Therapeutics, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
  • Dhennezel C; Department of Chemistry, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
  • Pishchany G; Center for Microbiome Informatics and Therapeutics, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
  • Lensch V; Department of Chemistry, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
  • Vlamakis H; Center for Microbiome Informatics and Therapeutics, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
  • Alm EJ; Department of Bacteriology, University of Wisconsin-Madison, Madison, WI 53706, USA.
  • Xavier RJ; Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
  • Kiessling LL; Department of Bacteriology, University of Wisconsin-Madison, Madison, WI 53706, USA.
Sci Adv ; 9(30): eadd8766, 2023 07 28.
Article en En | MEDLINE | ID: mdl-37506208
Soluble human lectins are critical components of innate immunity. Genetic models suggest that lectins influence host-resident microbiota, but their specificity for commensal and mutualist species is understudied. Elucidating lectins' roles in regulating microbiota requires an understanding of which microbial species they bind within native communities. To profile human lectin recognition, we developed Lectin-Seq. We apply Lectin-Seq to human fecal microbiota using the soluble mannose-binding lectin (MBL) and intelectin-1 (hItln1). Although each lectin binds a substantial percentage of the samples (10 to 20%), the microbial interactomes of MBL and hItln1 differ markedly in composition and diversity. MBL binding is highly selective for a small subset of species commonly associated with humans. In contrast, hItln1's interaction profile encompasses a broad range of lower-abundance species. Our data uncover stark differences in the commensal recognition properties of human lectins.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Inmunidad Innata / Lectinas Límite: Humans Idioma: En Revista: Sci Adv Año: 2023 Tipo del documento: Article País de afiliación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Inmunidad Innata / Lectinas Límite: Humans Idioma: En Revista: Sci Adv Año: 2023 Tipo del documento: Article País de afiliación: Estados Unidos