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NuMY-A qPCR Assay Simultaneously Targeting Human Autosomal, Y-Chromosomal, and Mitochondrial DNA.
Xavier, Catarina; Sutter, Charlotte; Amory, Christina; Niederstätter, Harald; Parson, Walther.
Afiliación
  • Xavier C; Institute of Legal Medicine, Medical University of Innsbruck, 6020 Innsbruck, Austria.
  • Sutter C; i3S-Institute for Research and Innovation in Health, University of Porto, 4099-002 Porto, Portugal.
  • Amory C; Institute of Legal Medicine, Medical University of Innsbruck, 6020 Innsbruck, Austria.
  • Niederstätter H; Institute of Legal Medicine, Medical University of Innsbruck, 6020 Innsbruck, Austria.
  • Parson W; Institute of Legal Medicine, Medical University of Innsbruck, 6020 Innsbruck, Austria.
Genes (Basel) ; 14(8)2023 08 18.
Article en En | MEDLINE | ID: mdl-37628695
ABSTRACT
The accurate quantification of DNA in forensic samples is of utmost importance. These samples are often present in limited amounts; therefore, it is indicated to use the appropriate analysis route with the optimum DNA amount (when possible). Also, DNA quantification can inform about the degradation stage and therefore support the decision on which downstream genotyping method to use. Consequently, DNA quantification aids in getting the best possible results from a forensic sample, considering both its DNA quantity and quality limitations. Here, we introduce NuMY, a new quantitative real-time PCR (qPCR) method for the parallel quantification of human nuclear (n) and mitochondrial (mt) DNA, assessing the male portion in mixtures of both sexes and testing for possible PCR inhibition. NuMY is based on previous work and follows the MIQE guidelines whenever applicable. Although quantification of nuclear (n)DNA by simultaneously analyzing autosomal and male-specific targets is available in commercial qPCR kits, tools that include the quantification of mtDNA are sparse. The quantification of mtDNA has proven relevant for samples with low nDNA content when conventional DNA fingerprinting techniques cannot be followed. Furthermore, the development and use of new massively parallel sequencing assays that combine multiple marker types, i.e., autosomal, Y-chromosomal, and mtDNA, can be optimized when precisely knowing the amount of each DNA component present in the input sample. For high-quality DNA extracts, NuMY provided nDNA results comparable to those of another quantification technique and has also proven to be a reliable tool for challenging, forensically relevant samples such as mixtures, inhibited, and naturally degraded samples.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: ADN Mitocondrial / Mitocondrias Límite: Female / Humans / Male Idioma: En Revista: Genes (Basel) Año: 2023 Tipo del documento: Article País de afiliación: Austria

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: ADN Mitocondrial / Mitocondrias Límite: Female / Humans / Male Idioma: En Revista: Genes (Basel) Año: 2023 Tipo del documento: Article País de afiliación: Austria
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