Human platelet lysate improves the growth and survival of cultured human pre-antral follicles.
Reprod Biomed Online
; 47(5): 103256, 2023 11.
Article
en En
| MEDLINE
| ID: mdl-37690342
ABSTRACT
RESEARCH QUESTION How do platelet-rich plasma products like human platelet lysate (HPL) and umbilical cord plasma (UCP) affect the growth and survival of isolated human pre-antral follicles in vitro? DESIGN:
Human pre-antral follicles (nâ¯=â¯724; mean diameter 75 µm; range 46-237 µm) were isolated from ovarian medulla donated by 14 patients undergoing unilateral oophorectomy for ovarian tissue cryopreservation. Follicles were encapsulated in 0.5% alginate and cultured for 8 days in media supplemented with 5% fetal bovine serum (FBS) (nâ¯=â¯171), 2.5% human serum albumin (HSA) (nâ¯=â¯159), 5% HPL (nâ¯=â¯223) or 5% UCP (nâ¯=â¯171).RESULTS:
The survival probability was significantly higher in the group supplemented with HPL (80%) compared with the other three groups FBS (54%, P < 0.001); HSA (63%, Pâ¯=â¯0.004) and UCP (29%, P < 0.001). Surviving follicles in the UCP group had less defined follicular membranes and decompacted granulosa cell layers. The median growth of surviving follicles was significantly (P < 0.001) larger in the HPL group (73 µm) compared with any of the other three groups HSA (43 µm); FBS (40 µm) UCP (54 µm). A descriptive analysis of follicular secretion of anti-Müllerian hormone and oestradiol did not reveal any difference between the groups. The detectability of follicular genes was high for AR (100%), AMHR2 (100%) and FSHR (76%), whereas few follicles expressed LHR (20%).CONCLUSION:
Human platelet lysate significantly improved survival and growth of cultured human pre-antral follicles compared with FBS, HSA and UCP. The use of HPL is a valuable improvement to culture human pre-antral follicles but further studies will have to prove whether the superiority of HPL translates into better quality oocytes.Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Oocitos
/
Folículo Ovárico
Límite:
Female
/
Humans
Idioma:
En
Revista:
Reprod Biomed Online
Asunto de la revista:
MEDICINA REPRODUTIVA
Año:
2023
Tipo del documento:
Article