Generation and Characterisation of Monoclonal Antibodies against Nairobi Sheep Disease Virus Nucleoprotein.
Viruses
; 15(9)2023 09 05.
Article
en En
| MEDLINE
| ID: mdl-37766282
ABSTRACT
Nairobi sheep disease (NSD), caused by the viral agent NSD virus (NSDV), is a haemorrhagic fever disease affecting and inducing high mortality in sheep and goat populations. NSDV belongs to the genus Orthonairovirus of the Nairoviridae family from the order Bunyavirales. Other viruses circulating in livestock such as Crimean-Congo haemorrhagic fever virus (CCHFV) and Dugbe virus (DUGV) are members of the same genus and are reported to share antigenic features. There are very few available materials to study NSDV infection both in vitro and in vivo. In the present work, we characterised two monoclonal antibodies generated in mice that recognise NSDV specifically but not CCHFV or DUGV, along with a potential use to define virus-infected cells, using flow cytometry. We believe this tool can be useful for research, but also NSDV diagnostics, especially through immunological staining.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Nairovirus
/
Virus de la Fiebre Hemorrágica de Crimea-Congo
/
Virus de la Enfermedad de los Ovinos de Nairobi
/
Trastornos Hemorrágicos
Límite:
Animals
Idioma:
En
Revista:
Viruses
Año:
2023
Tipo del documento:
Article
País de afiliación:
Reino Unido