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The R2R3-MYB transcription factor EVER controls the emission of petunia floral volatiles by regulating epicuticular wax biosynthesis in the petal epidermis.
Skaliter, Oded; Bednarczyk, Dominika; Shor, Ekaterina; Shklarman, Elena; Manasherova, Ekaterina; Aravena-Calvo, Javiera; Kerzner, Shane; Cna'ani, Alon; Jasinska, Weronika; Masci, Tania; Dvir, Gony; Edelbaum, Orit; Rimon, Ben; Brotman, Yariv; Cohen, Hagai; Vainstein, Alexander.
Afiliación
  • Skaliter O; Institute of Plant Sciences and Genetics in Agriculture, The Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Rehovot 76100, Israel.
  • Bednarczyk D; Institute of Plant Sciences and Genetics in Agriculture, The Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Rehovot 76100, Israel.
  • Shor E; Institute of Plant Sciences and Genetics in Agriculture, The Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Rehovot 76100, Israel.
  • Shklarman E; Institute of Plant Sciences and Genetics in Agriculture, The Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Rehovot 76100, Israel.
  • Manasherova E; Department of Vegetable and Field Crops, Institute of Plant Sciences, Agricultural Research Organization (ARO), Volcani Institute, Rishon LeZion 7505101, Israel.
  • Aravena-Calvo J; Institute of Plant Sciences and Genetics in Agriculture, The Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Rehovot 76100, Israel.
  • Kerzner S; Institute of Plant Sciences and Genetics in Agriculture, The Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Rehovot 76100, Israel.
  • Cna'ani A; Institute of Plant Sciences and Genetics in Agriculture, The Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Rehovot 76100, Israel.
  • Jasinska W; Department of Life Sciences, Ben-Gurion University of the Negev, Beer-Sheva 84105, Israel.
  • Masci T; Institute of Plant Sciences and Genetics in Agriculture, The Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Rehovot 76100, Israel.
  • Dvir G; Institute of Plant Sciences and Genetics in Agriculture, The Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Rehovot 76100, Israel.
  • Edelbaum O; Institute of Plant Sciences and Genetics in Agriculture, The Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Rehovot 76100, Israel.
  • Rimon B; Department of Ornamental Horticulture and Biotechnology, The Institute of Plant Sciences, Agricultural Research Organization, Volcani Institute, Rishon LeZion 7505101, Israel.
  • Brotman Y; Department of Life Sciences, Ben-Gurion University of the Negev, Beer-Sheva 84105, Israel.
  • Cohen H; Department of Vegetable and Field Crops, Institute of Plant Sciences, Agricultural Research Organization (ARO), Volcani Institute, Rishon LeZion 7505101, Israel.
  • Vainstein A; Institute of Plant Sciences and Genetics in Agriculture, The Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Rehovot 76100, Israel.
Plant Cell ; 36(1): 174-193, 2023 Dec 21.
Article en En | MEDLINE | ID: mdl-37818992
ABSTRACT
The epidermal cells of petunia (Petunia × hybrida) flowers are the main site of volatile emission. However, the mechanisms underlying the release of volatiles into the environment are still being explored. Here, using cell-layer-specific transcriptomic analysis, reverse genetics by virus-induced gene silencing and clustered regularly interspaced short palindromic repeat (CRISPR), and metabolomics, we identified EPIDERMIS VOLATILE EMISSION REGULATOR (EVER)-a petal adaxial epidermis-specific MYB activator that affects the emission of volatiles. To generate ever knockout lines, we developed a viral-based CRISPR/Cas9 system for efficient gene editing in plants. These knockout lines, together with transient-suppression assays, revealed EVER's involvement in the repression of low-vapor-pressure volatiles. Internal pools and annotated scent-related genes involved in volatile production and emission were not affected by EVER. RNA-Seq analyses of petals of ever knockout lines and EVER-overexpressing flowers revealed enrichment in wax-related biosynthesis genes. Liquid chromatography/gas chromatography-MS analyses of petal epicuticular waxes revealed substantial reductions in wax loads in ever petals, particularly of monomers of fatty acids and wax esters. These results implicate EVER in the emission of volatiles by fine-tuning the composition of petal epicuticular waxes. We reveal a petunia MYB regulator that interlinks epicuticular wax composition and volatile emission, thus unraveling a regulatory layer in the scent-emission machinery in petunia flowers.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Factores de Transcripción / Petunia Tipo de estudio: Prognostic_studies Idioma: En Revista: Plant Cell Asunto de la revista: BOTANICA Año: 2023 Tipo del documento: Article País de afiliación: Israel
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Factores de Transcripción / Petunia Tipo de estudio: Prognostic_studies Idioma: En Revista: Plant Cell Asunto de la revista: BOTANICA Año: 2023 Tipo del documento: Article País de afiliación: Israel