Identification and Localization of Labile Molecules in Broccoli Cell by CLA-ICP-MS and Single-Cell Nanospray HRMS.
J Am Soc Mass Spectrom
; 34(11): 2454-2460, 2023 Nov 01.
Article
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| MEDLINE
| ID: mdl-37830133
ABSTRACT
Although molecular analysis and imaging by mass spectrometry are emerging as tools to identify metabolites and determine their distribution in cells and tissues, it is difficult to directly analyze the labile molecules at the single-cell level. Glucosinolate (GL) is a plant-active substance with much attention as a chemical defense mechanism known as a "mustard oil bomb" in broccoli. When tissue is damaged, these substances undergo rapid degradation, making them unsuitable for conventional mass spectrometry (MS), particularly for surface MS imaging analysis methods that necessitate intricate preprocessing. Herein, a strategy combining cryogenic laser ablation inductively coupled mass spectrometry (CLA-ICP-MS) and capillary microsampling nanospray high-resolution mass spectrometry (HRMS) was developed. The sulfur-rich microzone in tissue which was thought as a suspect GL-rich cell population was located via CLA-ICP-MS. Three GLs in single cells were accurately identified by nanospray HRMS with a hydrogen/deuterium exchange reaction. Subsequently, cell-by-cell imaging by nanospray HRMS showed that the GL-rich cells were below the stalk surface by approximately 30 µm. This proposed strategy can also be applied to rapidly identify labile compounds and localize molecule-rich cells in tissues.
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1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Brassica
/
Terapia por Láser
Idioma:
En
Revista:
J Am Soc Mass Spectrom
Año:
2023
Tipo del documento:
Article