Acquisition site-based remodelling of Clostridium perfringens- and Clostridioides difficile-related gut microbiota.
Comp Immunol Microbiol Infect Dis
; 102: 102074, 2023 Nov.
Article
en En
| MEDLINE
| ID: mdl-37832162
ABSTRACT
INTRODUCTION:
Clostridium perfringens is a gram-positive, anaerobic sporulating bacillus which can infect several hosts, thereby being considered the causative agent of many gut illnesses. Some studies have suggested that C. perfringens's virulence factors may negatively affect gut microbiota homeostasis by decreasing beneficial bacteria; however, studies have failed to evaluate the simultaneous presence of other pathogenic bacteria, such as C. difficile (another sporulating bacillus known to play a role in gut microbiota imbalance). Conscious of the lack of compelling data, this work has ascertained how such microorganisms' coexistence can be associated with a variation in gut microbiota composition, compared to that of C. perfringens colonisation.METHODS:
PCR was thus used for identifying C. perfringens and C. difficile in 98 samples. Amplicon-based sequencing of 16S- and 18S-rRNA genes' V4 hypervariable region from such samples was used for determining the microbiota's taxonomical composition and diversity.RESULTS:
Small differences were observed in bacterial communities' taxonomic composition and diversity; such imbalance was mainly associated with groups having hospital-acquired diarrhoea.CONCLUSION:
The alterations reported herein may have been influenced by C. difficile and diarrhoea acquisition site, despite C. perfringens' ability to cause alterations in microbiota due to its virulence factors. Our findings highlight the need for a holistic view of gut microbiota.Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Contexto en salud:
3_ND
Problema de salud:
3_zoonosis
Asunto principal:
Clostridioides difficile
/
Infecciones por Clostridium
/
Microbioma Gastrointestinal
Límite:
Animals
Idioma:
En
Revista:
Comp Immunol Microbiol Infect Dis
Año:
2023
Tipo del documento:
Article
País de afiliación:
Colombia