Identification, Phytochemistry and Biological Activities of Paris polyphylla on Hepatocellular Carcinoma.
Pak J Biol Sci
; 26(5): 203-212, 2023 Apr.
Article
en En
| MEDLINE
| ID: mdl-37859552
ABSTRACT
<b>Background and Objective:
</b> Liver cancer is the common cause of cancer death. <i>Paris polyphylla</i> is used as a traditional folk medicine in Vietnam to treat pneumonia, mastitis, bruises and fractures but no study was available regarding its ability to treat liver cancer or slow its growth. In this study, <i>Paris polyphylla</i> samples were identified and evaluated cytotoxic activity against the liver cancer cells. <b>Materials andMethods:
</b> <i>Paris polyphylla</i> species were collected from various areas in Yen Bai, Vietnam, which were identified by comparative morphological method and DNA barcoding for the <i>18S, matK</i> genes and <i>ITS</i> region. <i>Paris polyphylla</i> samples were dried until constant weight, ground into a fine powder and extracted in various solvents. The bioactivity of these extracts were done by the MTT assay. <b>Results:
</b> The sequences of <i>18S, matK</i> genes and <i>ITS</i> region were high similarity to sequences of <i>P. polyphylla</i> in the National Center for Biotechnology Information. The N-hexane and ethyl acetate fractions were produced from the methanol extract of <i>P. polyphylla</i>. The TLC results showed that there was a significant difference in the component of n-hexane and ethyl acetate fraction. The N-hexane fraction contains mainly low-polarity and non-polarity substances. While ethyl acetate fraction consists mainly of polar substances. In addition, ethyl acetate fraction was shown the strongest cytotoxic activity on the cancer cell lines HepG2 and Huh7 with the evaluation of IC<sub>50</sub> = 115.11±2.77 µg mL<sup>1</sup> and IC<sub>50</sub> = 148.11±1.78 µg mL<sup>1</sup>. <b>Conclusion:
</b> The extract of <i>Paris polyphylla</i> demonstrated strong potential to inhibit the growth of the liver cancer cell line. The ethyl acetate fraction has the highest ability for cytotoxicity on the liver and cell line at a concentration of 200 µg mL<sup>1</sup> through MTT.Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Escarabajos
/
Carcinoma Hepatocelular
/
Liliaceae
/
Neoplasias Hepáticas
Límite:
Animals
Idioma:
En
Revista:
Pak J Biol Sci
Año:
2023
Tipo del documento:
Article