Automated Platform for the Plasmid Construction Process.
ACS Synth Biol
; 12(12): 3506-3513, 2023 Dec 15.
Article
en En
| MEDLINE
| ID: mdl-37948662
ABSTRACT
There is a growing need for applications capable of handling large synthesis biology experiments. At the core of synthetic biology is the process of cloning and manipulating DNA as plasmids. Here, we report the development of an application named DNAda capable of writing automation instructions for any given DNA construct design generated by the J5 DNA assembly program. We also describe the automation pipeline and several useful features. The pipeline is particularly useful for the construction of combinatorial DNA assemblies. Furthermore, we demonstrate the platform by constructing a library of polyketide synthase parts, which includes 120 plasmids ranging in size from 7 to 14 kb from 4 to 7 DNA fragments.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
ADN
/
Biología Sintética
Idioma:
En
Revista:
ACS Synth Biol
Año:
2023
Tipo del documento:
Article
País de afiliación:
Estados Unidos