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Eukaryotic RNA-guided endonucleases evolved from a unique clade of bacterial enzymes.
Yoon, Peter H; Skopintsev, Petr; Shi, Honglue; Chen, LinXing; Adler, Benjamin A; Al-Shimary, Muntathar; Craig, Rory J; Loi, Kenneth J; DeTurk, Evan C; Li, Zheng; Amerasekera, Jasmine; Trinidad, Marena; Nisonoff, Hunter; Chen, Kai; Lahiri, Arushi; Boger, Ron; Jacobsen, Steve; Banfield, Jillian F; Doudna, Jennifer A.
Afiliación
  • Yoon PH; Department of Molecular and Cell Biology, University of California, Berkeley; Berkeley, CA, USA.
  • Skopintsev P; Innovative Genomics Institute; University of California, Berkeley, CA, USA.
  • Shi H; Innovative Genomics Institute; University of California, Berkeley, CA, USA.
  • Chen L; California Institute for Quantitative Biosciences (QB3), University of California, Berkeley, CA, USA.
  • Adler BA; Innovative Genomics Institute; University of California, Berkeley, CA, USA.
  • Al-Shimary M; Howard Hughes Medical Institute, University of California, Berkeley; Berkeley, CA, USA.
  • Craig RJ; Innovative Genomics Institute; University of California, Berkeley, CA, USA.
  • Loi KJ; Department of Earth and Planetary Science, University of California, Berkeley, CA, USA.
  • DeTurk EC; Innovative Genomics Institute; University of California, Berkeley, CA, USA.
  • Li Z; California Institute for Quantitative Biosciences (QB3), University of California, Berkeley, CA, USA.
  • Amerasekera J; Department of Molecular and Cell Biology, University of California, Berkeley; Berkeley, CA, USA.
  • Trinidad M; Innovative Genomics Institute; University of California, Berkeley, CA, USA.
  • Nisonoff H; California Institute for Quantitative Biosciences (QB3), University of California, Berkeley, CA, USA.
  • Chen K; Department of Molecular and Cell Biology, University of California, Berkeley; Berkeley, CA, USA.
  • Lahiri A; Innovative Genomics Institute; University of California, Berkeley, CA, USA.
  • Boger R; Innovative Genomics Institute; University of California, Berkeley, CA, USA.
  • Jacobsen S; California Institute for Quantitative Biosciences (QB3), University of California, Berkeley, CA, USA.
  • Banfield JF; Department of Molecular, Cell and Developmental Biology, University of California, Los Angeles, CA, USA.
  • Doudna JA; Department of Human Genetics, University of California, Los Angeles, CA, USA.
Nucleic Acids Res ; 51(22): 12414-12427, 2023 Dec 11.
Article en En | MEDLINE | ID: mdl-37971304
RNA-guided endonucleases form the crux of diverse biological processes and technologies, including adaptive immunity, transposition, and genome editing. Some of these enzymes are components of insertion sequences (IS) in the IS200/IS605 and IS607 transposon families. Both IS families encode a TnpA transposase and a TnpB nuclease, an RNA-guided enzyme ancestral to CRISPR-Cas12s. In eukaryotes, TnpB homologs occur as two distinct types, Fanzor1s and Fanzor2s. We analyzed the evolutionary relationships between prokaryotic TnpBs and eukaryotic Fanzors, which revealed that both Fanzor1s and Fanzor2s stem from a single lineage of IS607 TnpBs with unusual active site arrangement. The widespread nature of Fanzors implies that the properties of this particular lineage of IS607 TnpBs were particularly suited to adaptation in eukaryotes. Biochemical analysis of an IS607 TnpB and Fanzor1s revealed common strategies employed by TnpBs and Fanzors to co-evolve with their cognate transposases. Collectively, our results provide a new model of sequential evolution from IS607 TnpBs to Fanzor2s, and Fanzor2s to Fanzor1s that details how genes of prokaryotic origin evolve to give rise to new protein families in eukaryotes.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Bacterias / Evolución Molecular / Endonucleasas Idioma: En Revista: Nucleic Acids Res Año: 2023 Tipo del documento: Article País de afiliación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Bacterias / Evolución Molecular / Endonucleasas Idioma: En Revista: Nucleic Acids Res Año: 2023 Tipo del documento: Article País de afiliación: Estados Unidos