Selecting mRNA markers in blood for age estimation of the donor of a biological stain.
Forensic Sci Int Genet
; 68: 102976, 2024 01.
Article
en En
| MEDLINE
| ID: mdl-38000161
ABSTRACT
RNA has gained a substantial amount of attention within the forensic field over the last decade. There is evidence that RNAs are differentially expressed with biological age. Since RNA can be co-extracted with DNA from the same piece of evidence, RNA-based analysis appears as a promising molecular alternative for predicting the biological age and hence inferring the chronological age of a person. Using RNA-Seq data we searched for markers in blood potentially associated with age. We used our own RNA-Seq data from dried blood stains as well as publicly available RNA-Seq data from whole blood, and compared two different approaches to select candidate markers. The first approach focused on individual gene analysis with DESeq2 to select the genes most correlated with age, while the second approach employed lasso regression to select a set of genes for optimal prediction of age. We present two lists with 270 candidate markers, one for each approach.
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Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
ADN
/
Colorantes
Límite:
Humans
Idioma:
En
Revista:
Forensic Sci Int Genet
Asunto de la revista:
GENETICA
/
JURISPRUDENCIA
Año:
2024
Tipo del documento:
Article
País de afiliación:
Suiza