3D structured illumination microscope using a spinning disk [Invited].
Biomed Opt Express
; 14(11): 5710-5719, 2023 Nov 01.
Article
en En
| MEDLINE
| ID: mdl-38021136
ABSTRACT
Three-dimensional (3D) structured illumination microscopy (SIM) improves spatial resolution by a factor of two in both lateral and axial directions. However, the adoption of 3D SIM is limited by low imaging speed, susceptibility to out-of-focus light, and likelihood of reconstruction errors. Here we present a novel approach for 3D SIM using a spinning disk. The disk generates a 3D lattice illumination pattern on the sample and optically reconstructs super-resolved images in real time. This technique achieves a 2-times resolution improvement with a speed up to 100 frames per second while physically rejecting 90% of the background signal.
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1
Colección:
01-internacional
Base de datos:
MEDLINE
Idioma:
En
Revista:
Biomed Opt Express
Año:
2023
Tipo del documento:
Article
País de afiliación:
Canadá