Ratiometric fluorescence sensing and quantification of circulating blood sodium sensors in mice in vivo.
Biomed Opt Express
; 14(11): 5555-5568, 2023 Nov 01.
Article
en En
| MEDLINE
| ID: mdl-38021147
ABSTRACT
In this work, we introduce ratiometric diffuse in vivo flow cytometry (R-DiFC) for quantitative measurement of circulating fluorescent red blood cell (fRBC) sensors for systemic blood sodium levels. Unlike in our previous work in measuring circulating fRBC sensors, R-DiFC allows simultaneous measurement of two fluorophores encapsulated in the sensor, the ratio of which enables self-calibration of the fluorescence signal with different fRBC depths in biological tissue. We show that the R-DiFC signal varies significantly less than either fluorescence signal alone. This work holds promise for personalized monitoring of systemic sodium for bipolar patients in the future.
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1
Colección:
01-internacional
Base de datos:
MEDLINE
Idioma:
En
Revista:
Biomed Opt Express
Año:
2023
Tipo del documento:
Article
País de afiliación:
Estados Unidos