Co-factor prosthesis facilitates biosynthesis of azido-pseudaminic acid probes for use as glycosyltransferase reporters.

Keenan, Tessa; Chidwick, Harriet S; Best, Matthew; Flack, Emily K P; Yates, Nicholas D J; Hatton, Natasha E; Warnes, Matthew E; Fascione, Martin A

Keenan, Tessa; Chidwick, Harriet S; Best, Matthew; Flack, Emily K P; Yates, Nicholas D J; Hatton, Natasha E; Warnes, Matthew E; Fascione, Martin A.

Afiliación

Keenan T; Department of Chemistry, University of York, Heslington, York, YO10 5DD, UK. martin.fascione@york.ac.uk.
Chidwick HS; Department of Chemistry, University of York, Heslington, York, YO10 5DD, UK. martin.fascione@york.ac.uk.
Best M; Department of Chemistry, University of York, Heslington, York, YO10 5DD, UK. martin.fascione@york.ac.uk.
Flack EKP; Department of Chemistry, University of York, Heslington, York, YO10 5DD, UK. martin.fascione@york.ac.uk.
Yates NDJ; Department of Biology, University of York, York, YO10 5DD, UK.
Hatton NE; Department of Chemistry, University of York, Heslington, York, YO10 5DD, UK. martin.fascione@york.ac.uk.
Warnes ME; Department of Chemistry, University of York, Heslington, York, YO10 5DD, UK. martin.fascione@york.ac.uk.
Fascione MA; Department of Chemistry, University of York, Heslington, York, YO10 5DD, UK. martin.fascione@york.ac.uk.

Chem Commun (Camb) ; 60(11): 1428-1431, 2024 Feb 01.

Article en En | MEDLINE | ID: mdl-38205715

ABSTRACT

ABSTRACT

Truncated thioester N,S-diacetylcysteamine (SNAc) was utilised as a co-factor mimic for PseH, an acetyl-coA dependent aminoglycoside N-acetyltransferase, in the biosynthesis of the bacterial sugar, pseudaminic acid. Additionally, an azido-SNAc analogue was used to smuggle N7-azide functionality into the pseudaminic acid backbone, facilitating its use as a reporter of pseudaminyltransferase activity.

Asunto(s)

Glicosiltransferasas; Azúcares Ácidos; Prótesis e Implantes

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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Azúcares Ácidos / Glicosiltransferasas Idioma: En Revista: Chem Commun (Camb) Asunto de la revista: QUIMICA Año: 2024 Tipo del documento: Article País de afiliación: Reino Unido

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