Screening for the production of polyunsaturated fatty acids and cerebrosides in fungi.
J Appl Microbiol
; 135(2)2024 Feb 01.
Article
en En
| MEDLINE
| ID: mdl-38323436
ABSTRACT
AIMS:
To investigate fatty acid, including polyunsaturated fatty acids (PUFA), and cerebroside production of a large diversity of fungi from the Ascomycota, Basidiomycota, and Mucoromycota phyla. METHODS ANDRESULTS:
Seventy-nine fungal strains were grown in Kavadia medium using a microcultivation system, i.e. Duetz microtiter plates. Following cultivation, fatty acid and cerebroside contents were analyzed by gas chromatography-flame ionization detection (GC-FID) and high performance thin-layer chromatography (HPTLC), respectively. Mucoromycota fungi appeared as the most promising candidates for omega-6 PUFA production. The best omega-6 producer, including γ-linolenic acid (GLA, 183n-6), was Mucor fragilis UBOCC-A109196 with a concentration of 647 mg L-1 total omega-6 PUFA (representing 35% of total fatty acids) and 225 mg L-1 GLA (representing 12% of total fatty acids). Arachidonic acid concentration (204n-6) was the highest in Mortierella alpina UBOCC-A-112046, reaching 255 mg L-1 and 18.56% of total fatty acids. Interestingly, several fungal strains were shown to produce omega-7 monounsaturated fatty acids. Indeed, Torulaspora delbrueckii strains accumulated palmitoleic acid (161n-7) up to 20% of total fatty acids, reaching 114 mg L-1 in T. delbrueckii UBOCC-A-214128, while C. elegans UBOCC-A-102008 produced mainly paullinic acid (201n-7) with concentrations up to 100 mg L-1. Concerning cerebroside production, HPTLC appeared as a relevant approach for their detection and quantification. Promising candidates belonging to the Mucoromycota phylum were found, especially in the Absidia genus with A. spinosa UBOCC-A-101332 as the best producer (12.7 mg L-1).CONCLUSIONS:
The present study highlighted PUFA and cerebroside production in a large diversity of fungi and the fact that members of the Mucoromycota phylum are good producers of PUFA as well as cerebrosides.Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Caenorhabditis elegans
/
Ácidos Grasos Insaturados
Tipo de estudio:
Diagnostic_studies
/
Screening_studies
Límite:
Animals
Idioma:
En
Revista:
J Appl Microbiol
Asunto de la revista:
MICROBIOLOGIA
Año:
2024
Tipo del documento:
Article
País de afiliación:
Francia