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Immunogenic and Protective Properties of Recombinant Hemagglutinin of Influenza A (H5N8) Virus.
Rudometova, Nadezhda B; Fando, Anastasia A; Kisakova, Lyubov A; Kisakov, Denis N; Borgoyakova, Mariya B; Litvinova, Victoria R; Yakovlev, Vladimir A; Tigeeva, Elena V; Vahitov, Danil I; Sharabrin, Sergey V; Shcherbakov, Dmitriy N; Evseenko, Veronika I; Ivanova, Ksenia I; Gudymo, Andrei S; Ilyicheva, Tatiana N; Marchenko, Vasiliy Yu; Ilyichev, Alexander A; Rudometov, Andrey P; Karpenko, Larisa I.
Afiliación
  • Rudometova NB; Federal Budgetary Research Institution State Research Center of Virology and Biotechnology «Vector¼, Rospotrebnadzor, Koltsovo 630559, Novosibirsk Region, Russia.
  • Fando AA; Federal Budgetary Research Institution State Research Center of Virology and Biotechnology «Vector¼, Rospotrebnadzor, Koltsovo 630559, Novosibirsk Region, Russia.
  • Kisakova LA; Federal Budgetary Research Institution State Research Center of Virology and Biotechnology «Vector¼, Rospotrebnadzor, Koltsovo 630559, Novosibirsk Region, Russia.
  • Kisakov DN; Federal Budgetary Research Institution State Research Center of Virology and Biotechnology «Vector¼, Rospotrebnadzor, Koltsovo 630559, Novosibirsk Region, Russia.
  • Borgoyakova MB; Federal Budgetary Research Institution State Research Center of Virology and Biotechnology «Vector¼, Rospotrebnadzor, Koltsovo 630559, Novosibirsk Region, Russia.
  • Litvinova VR; Federal Budgetary Research Institution State Research Center of Virology and Biotechnology «Vector¼, Rospotrebnadzor, Koltsovo 630559, Novosibirsk Region, Russia.
  • Yakovlev VA; Federal Budgetary Research Institution State Research Center of Virology and Biotechnology «Vector¼, Rospotrebnadzor, Koltsovo 630559, Novosibirsk Region, Russia.
  • Tigeeva EV; Federal Budgetary Research Institution State Research Center of Virology and Biotechnology «Vector¼, Rospotrebnadzor, Koltsovo 630559, Novosibirsk Region, Russia.
  • Vahitov DI; Federal Budgetary Research Institution State Research Center of Virology and Biotechnology «Vector¼, Rospotrebnadzor, Koltsovo 630559, Novosibirsk Region, Russia.
  • Sharabrin SV; Federal Budgetary Research Institution State Research Center of Virology and Biotechnology «Vector¼, Rospotrebnadzor, Koltsovo 630559, Novosibirsk Region, Russia.
  • Shcherbakov DN; Federal Budgetary Research Institution State Research Center of Virology and Biotechnology «Vector¼, Rospotrebnadzor, Koltsovo 630559, Novosibirsk Region, Russia.
  • Evseenko VI; Institute of Solid State Chemistry and Mechanochemistry, Siberian Branch of Russian Academy of Sciences, Novosibirsk 630090, Novosibirsk Region, Russia.
  • Ivanova KI; Federal Budgetary Research Institution State Research Center of Virology and Biotechnology «Vector¼, Rospotrebnadzor, Koltsovo 630559, Novosibirsk Region, Russia.
  • Gudymo AS; Federal Budgetary Research Institution State Research Center of Virology and Biotechnology «Vector¼, Rospotrebnadzor, Koltsovo 630559, Novosibirsk Region, Russia.
  • Ilyicheva TN; Federal Budgetary Research Institution State Research Center of Virology and Biotechnology «Vector¼, Rospotrebnadzor, Koltsovo 630559, Novosibirsk Region, Russia.
  • Marchenko VY; Federal Budgetary Research Institution State Research Center of Virology and Biotechnology «Vector¼, Rospotrebnadzor, Koltsovo 630559, Novosibirsk Region, Russia.
  • Ilyichev AA; Federal Budgetary Research Institution State Research Center of Virology and Biotechnology «Vector¼, Rospotrebnadzor, Koltsovo 630559, Novosibirsk Region, Russia.
  • Rudometov AP; Federal Budgetary Research Institution State Research Center of Virology and Biotechnology «Vector¼, Rospotrebnadzor, Koltsovo 630559, Novosibirsk Region, Russia.
  • Karpenko LI; Federal Budgetary Research Institution State Research Center of Virology and Biotechnology «Vector¼, Rospotrebnadzor, Koltsovo 630559, Novosibirsk Region, Russia.
Vaccines (Basel) ; 12(2)2024 Jan 29.
Article en En | MEDLINE | ID: mdl-38400127
ABSTRACT
In this study, we characterized recombinant hemagglutinin (HA) of influenza A (H5N8) virus produced in Chinese hamster ovary cells (CHO-K1s). Immunochemical analysis showed that the recombinant hemagglutinin was recognized by the serum of ferrets infected with influenza A (H5N8) virus, indicating that its antigenic properties were retained. Two groups of Balb/c mice were immunized with intramuscular injection of recombinant hemagglutinin or propiolactone inactivated A/Astrakhan/3212/2020 (H5N8) influenza virus. The results demonstrated that both immunogens induced a specific antibody response as determined by ELISA. Virus neutralization assay revealed that sera of immunized animals were able to neutralize A/turkey/Stavropol/320-01/2020 (H5N8) influenza virus-the average neutralizing titer was 2560. Immunization with both recombinant HA/H5 hemagglutinin and inactivated virus gave 100% protection against lethal H5N8 virus challenge. This study shows that recombinant HA (H5N8) protein may be a useful antigen candidate for developing subunit vaccines against influenza A (H5N8) virus with suitable immunogenicity and protective efficacy.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Vaccines (Basel) Año: 2024 Tipo del documento: Article País de afiliación: Rusia
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Vaccines (Basel) Año: 2024 Tipo del documento: Article País de afiliación: Rusia