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Leveraging Cas13a's trans-cleavage on RNA G-quadruplexes for amplification-free RNA detection.
Li, Tao; Chen, Dongjuan; He, Xiaoling; Li, Zheyu; Xu, Zhichen; Li, Runchen; Zheng, Bingxin; Hu, Rui; Zhu, Jiang; Li, Ying; Yang, Yunhuang.
Afiliación
  • Li T; State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics, Key Laboratory of Magnetic Resonance in Biological Systems, National Center for Magnetic Resonance in Wuhan, Wuhan Institute of Physics and Mathematics, Innovation Academy for Precision Measurement Science and Technology-Wu
  • Chen D; University of Chinese Academy of Sciences, Beijing 100049, China.
  • He X; Department of Laboratory Medicine, Maternal and Child Health Hospital of Hubei Province, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430070, China.
  • Li Z; State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics, Key Laboratory of Magnetic Resonance in Biological Systems, National Center for Magnetic Resonance in Wuhan, Wuhan Institute of Physics and Mathematics, Innovation Academy for Precision Measurement Science and Technology-Wu
  • Xu Z; University of Chinese Academy of Sciences, Beijing 100049, China.
  • Li R; State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics, Key Laboratory of Magnetic Resonance in Biological Systems, National Center for Magnetic Resonance in Wuhan, Wuhan Institute of Physics and Mathematics, Innovation Academy for Precision Measurement Science and Technology-Wu
  • Zheng B; University of Chinese Academy of Sciences, Beijing 100049, China.
  • Hu R; State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics, Key Laboratory of Magnetic Resonance in Biological Systems, National Center for Magnetic Resonance in Wuhan, Wuhan Institute of Physics and Mathematics, Innovation Academy for Precision Measurement Science and Technology-Wu
  • Zhu J; University of Chinese Academy of Sciences, Beijing 100049, China.
  • Li Y; State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics, Key Laboratory of Magnetic Resonance in Biological Systems, National Center for Magnetic Resonance in Wuhan, Wuhan Institute of Physics and Mathematics, Innovation Academy for Precision Measurement Science and Technology-Wu
  • Yang Y; University of Chinese Academy of Sciences, Beijing 100049, China.
Chem Commun (Camb) ; 60(23): 3166-3169, 2024 Mar 14.
Article en En | MEDLINE | ID: mdl-38410041
ABSTRACT
In this study, we investigated Cas13a's efficacy in trans-cleaving RNA G-quadruplexes (rG4s) as an alternative to ssRNA reporters in CRISPR-Cas13a diagnostics. Our findings demonstrate enhanced efficiency due to the structural arrangement of rG4s. Implementing a simplified CRISPR-Cas13a system based on rG4, we identified SARS-CoV-2 infections in 25 patient samples within 1 hour without target pre-amplification.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: G-Cuádruplex / COVID-19 Límite: Humans Idioma: En Revista: Chem Commun (Camb) Asunto de la revista: QUIMICA Año: 2024 Tipo del documento: Article
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: G-Cuádruplex / COVID-19 Límite: Humans Idioma: En Revista: Chem Commun (Camb) Asunto de la revista: QUIMICA Año: 2024 Tipo del documento: Article