Your browser doesn't support javascript.
loading
Surveillance of SARS-CoV-2 in wastewater by quantitative PCR and digital PCR: a case study in Shijiazhuang city, Hebei province, China.
Chai, Xiaoru; Liu, Shiyou; Liu, Chao; Bai, Jiaxuan; Meng, Juntao; Tian, Hong; Han, Xu; Han, Guangyue; Xu, Xiangdong; Li, Qi.
Afiliación
  • Chai X; School of Public Health, Hebei Medical University, Shijiazhuang, People's Republic of China.
  • Liu S; Hebei Key Laboratory of Pathogens and Epidemiology of Infectious Diseases, Hebei Provincial Center for Disease Control and Prevention, Shijiazhuang, People's Republic of China.
  • Liu C; Shijiazhuang Qiaodong Sewage Treatment Plant, Shijiazhuang, People's Republic of China.
  • Bai J; School of Public Health, Hebei Medical University, Shijiazhuang, People's Republic of China.
  • Meng J; School of Public Health, Hebei Medical University, Shijiazhuang, People's Republic of China.
  • Tian H; School of Public Health, Hebei Medical University, Shijiazhuang, People's Republic of China.
  • Han X; Hebei Key Laboratory of Pathogens and Epidemiology of Infectious Diseases, Hebei Provincial Center for Disease Control and Prevention, Shijiazhuang, People's Republic of China.
  • Han G; Hebei Key Laboratory of Pathogens and Epidemiology of Infectious Diseases, Hebei Provincial Center for Disease Control and Prevention, Shijiazhuang, People's Republic of China.
  • Xu X; School of Public Health, Hebei Medical University, Shijiazhuang, People's Republic of China.
  • Li Q; Hebei Key Laboratory of Environment and Human Health, Shijiazhuang, People's Republic of China.
Emerg Microbes Infect ; 13(1): 2324502, 2024 Dec.
Article en En | MEDLINE | ID: mdl-38465692
ABSTRACT
In this study, we reported the first long-term monitoring of SARS-CoV-2 in wastewater in Mainland China from November 2021 to October 2023. The city of Shijiazhuang was employed for this case study. We developed a triple reverse transcription droplet digital PCR (RT-ddPCR) method using triple primer-probes for simultaneous detection of the N1 gene, E gene, and Pepper mild mottle virus (PMMoV) to achieve accurate quantification of SARS-CoV-2 RNA in wastewater. Both the RT-ddPCR method and the commercial multiplex reverse transcription quantitative polymerase chain reaction (RT-qPCR) method were implemented for the detection of SARS-CoV-2 in wastewater in Shijiazhuang City over a 24-month period. Results showed that SARS-CoV-2 was detected for the first time in the wastewater of Shijiazhuang City on 10 November 2022. The peak of COVID-19 cases occurred in the middle of December 2022, when the concentration of SARS-CoV-2 in the wastewater was highest. The trend of virus concentration increases and decreases forming a "long-tailed" shape in the COVID-19  outbreak and recession cycle. The results indicated that both multiplex RT-ddPCR and RT-qPCR are effective in detecting SARS-CoV-2 in wastewater, but RT-ddPCR is capable of detecting low concentrations of SARS-CoV-2 in wastewater which is more efficient. The SARS-CoV-2 abundance in wastewater is correlated to clinical data, outlining the public health utility of this work.HighlightsFirst long-term monitoring of SARS-CoV-2 in wastewater in Mainland ChinaCOVID-19 outbreak was tracked in Shijiazhuang City from outbreak to containmentWastewater was monitored simultaneously using RT-ddPCR and RT-qPCR methodsTriple primer-probe RT-ddPCR detects N1 and E genes of SARS-CoV-2 and PMMoV.
Asunto(s)
Palabras clave

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Tobamovirus / SARS-CoV-2 / COVID-19 Límite: Humans País/Región como asunto: Asia Idioma: En Revista: Emerg Microbes Infect Año: 2024 Tipo del documento: Article

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Tobamovirus / SARS-CoV-2 / COVID-19 Límite: Humans País/Región como asunto: Asia Idioma: En Revista: Emerg Microbes Infect Año: 2024 Tipo del documento: Article
...