The characterization of protein lactylation in relation to cardiac metabolic reprogramming in neonatal mouse hearts.
J Genet Genomics
; 51(7): 735-748, 2024 Jul.
Article
en En
| MEDLINE
| ID: mdl-38479452
ABSTRACT
In mammals, the neonatal heart can regenerate upon injury within a short time after birth, while adults lose this ability. Metabolic reprogramming has been demonstrated to be critical for cardiomyocyte proliferation in the neonatal heart. Here, we reveal that cardiac metabolic reprogramming could be regulated by altering global protein lactylation. By performing 4D label-free proteomics and lysine lactylation (Kla) omics analyses in mouse hearts at postnatal days 1, 5, and 7, 2297 Kla sites from 980 proteins are identified, among which 1262 Kla sites from 409 proteins are quantified. Functional clustering analysis reveals that the proteins with altered Kla sites are mainly involved in metabolic processes. The expression and Kla levels of proteins in glycolysis show a positive correlation while a negative correlation in fatty acid oxidation. Furthermore, we verify the Kla levels of several differentially modified proteins, including ACAT1, ACADL, ACADVL, PFKM, PKM, and NPM1. Overall, our study reports a comprehensive Kla map in the neonatal mouse heart, which will help to understand the regulatory network of metabolic reprogramming and cardiac regeneration.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Proteómica
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Animales Recién Nacidos
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Miocardio
Límite:
Animals
Idioma:
En
Revista:
J Genet Genomics
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J. genet. genomics
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Journal of genetics and genomics
Año:
2024
Tipo del documento:
Article
País de afiliación:
China